Doxorubicin Affects Expression of Proteins of Neuronal Pathways in MCF-7 Breast Cancer Cells.

Petrovic, Marian; Simillion, Cedric André Marie; Kruzliak, Peter; Sabo, Jan; Heller, Manfred (2015). Doxorubicin Affects Expression of Proteins of Neuronal Pathways in MCF-7 Breast Cancer Cells. Cancer genomics & proteomics, 12(6), pp. 347-358. International Institute of Anticancer Research

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In the present article, we report on the semi-quantitative proteome analysis and related changes in protein expression of the MCF-7 breast cancer cell line following treatment with doxorubicin, using the precursor acquisition independent from ion count (PAcIFIC) mass spectrometry method. PAcIFIC represents a cost-effective and easy-to-use proteomics approach, enabling for deep proteome sequencing with minimal sample handling. The acquired proteomic data sets were searched for regulated Reactome pathways and Gene Ontology annotation terms using a new algorithm (SetRank). Using this approach, we identified pathways with significant changes (≤0.05), such as chromatin organization, DNA binding, embryo development, condensed chromosome, sequence-specific DNA binding, response to oxidative stress and response to toxin, as well as others. These sets of pathways are already well-described as being susceptible to chemotherapeutic drugs. Additionally, we found pathways related to neuron development, such as central nervous system neuron differentiation, neuron projection membrane and SNAP receptor activity. These later pathways might indicate biological mechanisms on the molecular level causing the known side-effect of doxorubicin chemotherapy, characterized as cognitive impairment, also called 'chemo brain'. Mass spectrometry data are available via ProteomeXchange with identifier PXD002998.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Protein- und Zellbiologie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR)

UniBE Contributor:

Petrovic, Marian; Simillion, Cedric André Marie and Heller, Manfred

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1790-6245

Publisher:

International Institute of Anticancer Research

Language:

English

Submitter:

Verena de Serra Frazao-Bill

Date Deposited:

12 Apr 2016 16:34

Last Modified:

12 Apr 2016 16:34

PubMed ID:

26543081

Uncontrolled Keywords:

Doxorubicin; LC-MS/MS; PAcIFIC; breast cancer; chemo brain; chemotherapy; neuronal pathway down-regulation; pathway analysis; side-effects

BORIS DOI:

10.7892/boris.80764

URI:

https://boris.unibe.ch/id/eprint/80764

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