Citrullination in the periodontium-a possible link between periodontitis and rheumatoid arthritis

Laugisch, Oliver; Wong, Alicia; Sroka, Aneta; Kantyka, Tomasz; Koziel, Joanna; Neuhaus, Klaus; Sculean, Anton; Venables, Patrick J; Potempa, Jan; Möller, Burkhard; Eick, Sigrun (2016). Citrullination in the periodontium-a possible link between periodontitis and rheumatoid arthritis. Clinical oral investigations, 20(4), pp. 675-683. Springer 10.1007/s00784-015-1556-7

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OBJECTIVES The aim of the present study was to assess human and bacterial peptidylarginine deiminase (PAD) activity in the gingival crevicular fluid (GCF) in the context of serum levels of antibodies against citrullinated epitopes in rheumatoid arthritis and periodontitis. MATERIALS AND METHODS Human PAD and Porphyromonas gingivalis-derived enzyme (PPAD) activities were measured in the GCF of 52 rheumatoid arthritis (RA) patients (48 with periodontitis and 4 without) and 44 non-RA controls (28 with periodontitis and 16 without). Serum antibodies against citrullinated epitopes were measured by ELISA. Bacteria being associated with periodontitis were determined by nucleic-acid-based methods. RESULTS Citrullination was present in 26 (50 %) RA patients and 23 (48 %) controls. PAD and PPAD activities were detected in 36 (69 %) and 30 (58 %) RA patients, respectively, and in 30 (68 %) and 21 (50 %) controls, respectively. PPAD activity was higher in RA and non-RA patients with periodontitis than in those without (p = 0.038; p = 0.004), and was detected in 35 of 59 P. gingivalis-positive samples, and in 16 of 37 P. gingivalis-negative samples in association with high antibody levels against that species. CONCLUSIONS PAD and PPAD activities within the periodontium are elevated in RA and non-RA patients with periodontitis. PPAD secreted by P. gingivalis residing in epithelial cells may exert its citrullinating activity in distant regions of the periodontium or even distant tissues. CLINICAL RELEVANCE In periodontitis, the citrullination of proteins/peptides by human and bacterial peptidylarginine deiminases may generate antibodies after breaching immunotolerance in susceptible individuals.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
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04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Rheumatology, Clinical Immunology and Allergology

UniBE Contributor:

Laugisch, Oliver; Neuhaus, Klaus; Sculean, Anton; Möller, Burkhard and Eick, Sigrun

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1432-6981

Publisher:

Springer

Language:

English

Submitter:

Stefan Kuchen

Date Deposited:

14 Apr 2016 09:10

Last Modified:

14 Dec 2016 13:44

Publisher DOI:

10.1007/s00784-015-1556-7

PubMed ID:

26264638

BORIS DOI:

10.7892/boris.80953

URI:

https://boris.unibe.ch/id/eprint/80953

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