Placental expression of the angiogenic placental growth factor is stimulated by both aldosterone and simulated starvation

Eisele, Nicole; Albrecht, Christiane; Mistry, Hiten Dhirubhai; Dick, Bernhard; Baumann, Marc Ulrich; Surbek, Daniel; Currie, Gemma; Delles, Christian; Mohaupt, Markus; Escher, Geneviève; Gennari, Carine (2016). Placental expression of the angiogenic placental growth factor is stimulated by both aldosterone and simulated starvation. Placenta, 40, pp. 18-24. Elsevier 10.1016/j.placenta.2016.02.004

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Aldosterone is an important factor supporting placental growth and fetal development. Recently, expression of placental growth factor (PlGF) has been observed in response to aldosterone exposure in different models of atherosclerosis. Thus, we hypothesized that aldosterone up-regulates growth-adaptive angiogenesis in pregnancy, via increased placental PlGF expression. We followed normotensive pregnant women (n = 24) throughout pregnancy and confirmed these results in a second independent first trimester cohort (n = 36). Urinary tetrahydroaldosterone was measured by gas chromatography-mass spectrometry and corrected for creatinine. Circulating PlGF concentrations were determined by ELISA. Additionally, cultured cell lines, adrenocortical H295R and choriocarcinoma BeWo cells, as well as primary human third trimester trophoblasts were tested in vitro. PlGF serum concentrations positively correlated with urinary tetrahydroaldosterone corrected for creatinine in these two independent cohorts. This observation was not due to PlGF, which did not induce aldosterone production in cultured H295R cells. On the other hand, PlGF expression was specifically enhanced by aldosterone in the presence of forskolin (p < 0.01) in trophoblasts. A pronounced stimulation of PlGF expression was observed with reduced glucose concentrations simulating starvation (p < 0.001). In conclusion, aldosterone stimulates placental PlGF production, enhancing its availability during human pregnancy, a response amplified by reduced glucose supply. Given the crucial role of PlGF in maintaining a healthy pregnancy, these data support a key role of aldosterone for a healthy pregnancy outcome.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Nephrologie / Hypertonie
04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Nephrology and Hypertension
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Unit Childrens Hospital > Forschungsgruppe Pränatale Medizin
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine
04 Faculty of Medicine > Other Institutions > Teaching Staff, Faculty of Medicine

UniBE Contributor:

Eisele, Nicole; Albrecht, Christiane; Mistry, Hiten Dhirubhai; Dick, Bernhard; Baumann, Marc Ulrich; Surbek, Daniel; Mohaupt, Markus; Escher, Geneviève and Gennari, Carine

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

0143-4004

Publisher:

Elsevier

Language:

English

Submitter:

Barbara Järmann-Bangerter

Date Deposited:

08 Jun 2016 11:54

Last Modified:

08 Jun 2016 11:54

Publisher DOI:

10.1016/j.placenta.2016.02.004

PubMed ID:

27016778

Uncontrolled Keywords:

Aldosterone; Glucose; Placental growth factor; Pregnancy; Trophoblast

BORIS DOI:

10.7892/boris.80976

URI:

https://boris.unibe.ch/id/eprint/80976

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