Ross, Daniela; Altmann, Michael (2016). A fast protocol for purification of translating mRNAs. Methods, 107, pp. 57-62. Elsevier 10.1016/j.ymeth.2016.03.021
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We present a protocol that enables easy extraction of translating mRNAs by purification of tagged ribosomes and associated mRNAs. By studying three different examples of stress influenced genes, we demonstrate the effectiveness and compare our rapid method to an older protocol (Halbeisen et al., 2009).
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine |
UniBE Contributor: |
Ross, Daniela, Altmann, Michael |
Subjects: |
500 Science > 570 Life sciences; biology 600 Technology > 610 Medicine & health |
ISSN: |
1046-2023 |
Publisher: |
Elsevier |
Language: |
English |
Submitter: |
Barbara Franziska Järmann-Bangerter |
Date Deposited: |
08 Jun 2016 11:58 |
Last Modified: |
05 Dec 2022 14:55 |
Publisher DOI: |
10.1016/j.ymeth.2016.03.021 |
PubMed ID: |
27020892 |
Uncontrolled Keywords: |
GCN4; GRE1; HSF1; Ribosome purification; Translation under stress conditions |
BORIS DOI: |
10.7892/boris.80980 |
URI: |
https://boris.unibe.ch/id/eprint/80980 |
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