A fast protocol for purification of translating mRNAs

Ross, Daniela; Altmann, Michael (2016). A fast protocol for purification of translating mRNAs. Methods, 107, pp. 57-62. Elsevier 10.1016/j.ymeth.2016.03.021

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We present a protocol that enables easy extraction of translating mRNAs by purification of tagged ribosomes and associated mRNAs. By studying three different examples of stress influenced genes, we demonstrate the effectiveness and compare our rapid method to an older protocol (Halbeisen et al., 2009).

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Ross, Daniela and Altmann, Michael

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1046-2023

Publisher:

Elsevier

Language:

English

Submitter:

Barbara Järmann-Bangerter

Date Deposited:

08 Jun 2016 11:58

Last Modified:

08 Sep 2016 01:30

Publisher DOI:

10.1016/j.ymeth.2016.03.021

PubMed ID:

27020892

Uncontrolled Keywords:

GCN4; GRE1; HSF1; Ribosome purification; Translation under stress conditions

BORIS DOI:

10.7892/boris.80980

URI:

https://boris.unibe.ch/id/eprint/80980

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