Plant Adenosine 5′-phosphosulfate Reductase Is a Novel Iron-Sulfur Protein

Kopriva, Stanislav; Büchert, Thomas; Fritz, Günter; Suter, Marianne; Weber, Markus; Benda, Rüdiger; Schaller, Johann; Feller, Urs; Schürmann, Peter; Schünemann, Volker; Trautwein, Alfred X.; Kroneck, Peter M. H.; Brunold, Christian (2001). Plant Adenosine 5′-phosphosulfate Reductase Is a Novel Iron-Sulfur Protein. Journal of biological chemistry, 276(46), pp. 42881-42886. American Society for Biochemistry and Molecular Biology 10.1074/jbc.M107424200

Text JBC_276_42881.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (144kB)

Adenosine 5′-phosphosulfate reductase (APR) catalyzes the two-electron reduction of adenosine 5′-phosphosulfate to sulfite and AMP, which represents the key step of sulfate assimilation in higher plants. Recombinant APRs from both Lemna minorand Arabidopsis thaliana were overexpressed inEscherichia coli and isolated as yellow-brown proteins. UV-visible spectra of these recombinant proteins indicated the presence of iron-sulfur centers, whereas flavin was absent. This result was confirmed by quantitative analysis of iron and acid-labile sulfide, suggesting a 4Fe-4S cluster as the cofactor. EPR spectroscopy of freshly purified enzyme showed, however, only a minor signal at g = 2.01. Therefore, Mössbauer spectra of 57Fe-enriched APR were obtained at 4.2 K in magnetic fields of up to 7 tesla, which were assigned to a diamagnetic 4Fe-4S2+ cluster. This cluster was unusual because only three of the iron sites exhibited the same Mössbauer parameters. The fourth iron site gave, because of the bistability of the fit, a significantly smaller isomer shift or larger quadrupole splitting than the other three sites. Thus, plant assimilatory APR represents a novel type of adenosine 5′-phosphosulfate reductase with a 4Fe-4S center as the sole cofactor, which is clearly different from the dissimilatory adenosine 5′-phosphosulfate reductases found in sulfate reducing bacteria.

Interest & Impact

Downloads

14 since deposited on 25 Apr 2016
0 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item