translin Is Required for Metabolic Regulation of Sleep

Murakami, Kazuma; Yurgel, Maria E; Stahl, Bethany A; Masek, Pavel; Mehta, Aradhana; Heidker, Rebecca; Bollinger, Wesley; Gingras, Robert M; Kim, Young-Joon; Williams, Jack; Suter, Beat; DiAngelo, Justin R; Keene, Alex C (2016). translin Is Required for Metabolic Regulation of Sleep. Current Biology, 26(7), pp. 972-980. Cell Press 10.1016/j.cub.2016.02.013

[img] Text
Translin.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

Dysregulation of sleep or feeding has enormous health consequences. In humans, acute sleep loss is associated with increased appetite and insulin insensitivity, while chronically sleep-deprived individuals are more likely to develop obesity, metabolic syndrome, type II diabetes, and cardiovascular disease. Conversely, metabolic state potently modulates sleep and circadian behavior; yet, the molecular basis for sleep-metabolism interactions remains poorly understood. Here, we describe the identification of translin (trsn), a highly conserved RNA/DNA binding protein, as essential for starvation-induced sleep suppression. Strikingly, trsn does not appear to regulate energy stores, free glucose levels, or feeding behavior suggesting the sleep phenotype of trsn mutant flies is not a consequence of general metabolic dysfunction or blunted response to starvation. While broadly expressed in all neurons, trsn is transcriptionally upregulated in the heads of flies in response to starvation. Spatially restricted rescue or targeted knockdown localizes trsn function to neurons that produce the tachykinin family neuropeptide Leucokinin. Manipulation of neural activity in Leucokinin neurons revealed these neurons to be required for starvation-induced sleep suppression. Taken together, these findings establish trsn as an essential integrator of sleep and metabolic state, with implications for understanding the neural mechanism underlying sleep disruption in response to environmental perturbation.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Suter, Beat

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0960-9822

Publisher:

Cell Press

Funders:

[4] Swiss National Science Foundation
[80] Kanton Bern

Language:

English

Submitter:

Beat Suter

Date Deposited:

01 Jun 2016 13:59

Last Modified:

01 Jun 2016 13:59

Publisher DOI:

10.1016/j.cub.2016.02.013

PubMed ID:

27020744

BORIS DOI:

10.7892/boris.81105

URI:

https://boris.unibe.ch/id/eprint/81105

Actions (login required)

Edit item Edit item
Provide Feedback