High-resolution imaging of 2D outer membrane protein F (OmpF) crystals by atomic force microscopy

Fotiadis, Dimitrios; Müller, Daniel J (2013). High-resolution imaging of 2D outer membrane protein F (OmpF) crystals by atomic force microscopy. In: Schmidt-Krey, Ingeborg; Cheng, Yifan (eds.) Electron Crystallography of Soluble and Membrane Proteins. Methods in Molecular Biology: Vol. 955 (pp. 461-474). Humana Press 10.1007/978-1-62703-176-9_24

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In this chapter the methodological bases are provided to achieve subnanometer resolution on two-dimensional (2D) membrane protein crystals by atomic force microscopy (AFM). This is outlined in detail with the example of AFM studies of the outer membrane protein F (OmpF) from the bacterium Escherichia coli (E. coli). We describe in detail the high-resolution imaging of 2D OmpF crystals in aqueous solution and under near-physiological conditions. The topographs of OmpF, and stylus effects and artifacts encountered when imaging by AFM are discussed.

Item Type:

Book Section (Book Chapter)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Biochemistry and Molecular Medicine

UniBE Contributor:

Fotiadis, Dimitrios José

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1064-3745

ISBN:

978-1-62703-176-9

Series:

Methods in Molecular Biology

Publisher:

Humana Press

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:23

Last Modified:

04 Mar 2014 09:23

Publisher DOI:

10.1007/978-1-62703-176-9_24

PubMed ID:

23132075

URI:

https://boris.unibe.ch/id/eprint/8148 (FactScience: 213635)

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