Assessment of the expression of biomarkers of uremic inflammation in dogs with renal disease

Nentwig, Alice; Schweighauser, Ariane; Maissen-Villiger, Carla; Bruckmaier, Rupert; Zurbriggen, Andreas; van Dorland, Hendrika Anette; Francey, Thierry (2016). Assessment of the expression of biomarkers of uremic inflammation in dogs with renal disease. American journal of veterinary research, 77(2), pp. 218-224. American Veterinary Medical Association 10.2460/ajvr.77.2.218

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OBJECTIVE To assess the expression of inflammatory cytokines and enzymes in venous whole blood of dogs with impaired renal function attributable to various causes. ANIMALS 46 dogs with acute kidney injury (AKI), 8 dogs with chronic kidney disease (CKD), and 10 healthy dogs. PROCEDURES Dogs with AKI and CKD were prospectively enrolled during 2010 if they met inclusion criteria. Demographic and laboratory characteristics were evaluated for each dog, and expression of inflammatory cytokines (interleukin [IL]-1α, IL-1β, IL-8, tumor necrosis factor [TNF]-α, IL-10, and transforming growth factor [TGF]-β) and enzymes (inducible nitric oxide synthase [iNOS] and 5-lipoxygenase [5-LO]) was measured in venous whole blood obtained at initial evaluation. RESULTS Dogs with impaired renal function had markedly higher expression of the cytokines IL-1α, IL-1β, and TGF-β and the enzyme 5-LO, compared with expression in healthy dogs. Additionally, 17 of 46 AKI dogs (but none of the CKD dogs) had higher IL-8 mRNA expression and 3 of 8 CKD dogs (but only 2/46 AKI dogs) had higher TNF-α expression, compared with results for healthy dogs. No significant difference between renal disease groups was detected for inflammatory markers and laboratory variables, degree of azotemia, or cause of impaired renal function. CONCLUSIONS AND CLINICAL RELEVANCE In this study, expression of the cytokines IL-1α, IL-1β, and TGF-β and the enzyme 5-LO was clearly increased in dogs with renal disease, which suggested that these markers were part of an inflammatory response in animals with AKI or CKD. (Am J Vet Res 2016;77:218-224).

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic > Small Animal Clinic, Internal Medicine
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Small Animal Clinic
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Experimental Clinical Research
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Physiology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Nentwig, Alice; Schweighauser, Ariane; Maissen-Villiger, Carla; Bruckmaier, Rupert; Zurbriggen, Andreas; van Dorland, Hendrika Anette and Francey, Thierry

Subjects:

600 Technology > 630 Agriculture

ISSN:

0002-9645

Publisher:

American Veterinary Medical Association

Language:

English

Submitter:

Thierry Francey-Spicher

Date Deposited:

13 Mar 2017 13:45

Last Modified:

14 Mar 2017 22:56

Publisher DOI:

10.2460/ajvr.77.2.218

PubMed ID:

27027717

BORIS DOI:

10.7892/boris.82020

URI:

https://boris.unibe.ch/id/eprint/82020

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