Molecular Characterization and Risk Factors of Giardia duodenalis among School Children from La Habana, Cuba

Jerez Puebla, Luis Enrique; Núñez, Fidel A.; Martínez Silva, Isabel; Rojas Rivero, Lázara; Martínez González, Marta; Méndez Sutil, Yuliet; Ayllón Valdés, Lucía; Atencio Millán, Iraís; Müller, Norbert (2015). Molecular Characterization and Risk Factors of Giardia duodenalis among School Children from La Habana, Cuba. Journal of Parasitology Research, 2015, p. 378643. 10.1155/2015/378643

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Giardia duodenalis is considered the most common protozoan infecting humans worldwide. Molecular characterization of G. duodenalis isolates has revealed the existence of eight groups (assemblages A to H) which differ in their host distribution. A cross-sectional study was conducted in 639 children from La Habana between January and December 2013. Two assemblage-specific PCRs were carried out for the molecular characterization. The overall prevalence of Giardia infection was 11.9%. DNA from 63 of 76 (82.9%) samples was successfully amplified by PCR-tpi, while 58 from 76 (76.3%) were detected by PCRE1-HF. Similar results by both PCRs were obtained in 54 from 76 samples (71%). According to these analyses, assemblage B and mixed assemblages A + B account for most of the Giardia infections in the cohort of children tested. Our current study identified assemblage B as predominant genotype in children infected with Giardia. Univariate analysis indicated that omission of washing hands before eating and keeping dogs at home were significant risk factors for a Giardia infection. In the future, novel molecular tools for a better discrimination of assemblages at the subassemblages level are needed to verify possible correlations between Giardia genotypes and symptomatology of giardiasis.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Parasitology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)

UniBE Contributor:

Müller, Norbert

Subjects:

600 Technology > 630 Agriculture

ISSN:

2090-0023

Language:

English

Submitter:

Norbert Müller

Date Deposited:

17 Jun 2016 11:45

Last Modified:

17 Aug 2018 08:04

Publisher DOI:

10.1155/2015/378643

PubMed ID:

26693345

BORIS DOI:

10.7892/boris.82123

URI:

https://boris.unibe.ch/id/eprint/82123

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