Deep resequencing of GWAS loci identifies independent rare variants associated with inflammatory bowel disease

Rivas, Manuel A; Beaudoin, Mélissa; Gardet, Agnes; Stevens, Christine; Sharma, Yashoda; Zhang, Clarence K; Boucher, Gabrielle; Ripke, Stephan; Ellinghaus, David; Burtt, Noel; Fennell, Tim; Kirby, Andrew; Latiano, Anna; Goyette, Philippe; Green, Todd; Halfvarson, Jonas; Haritunians, Talin; Korn, Joshua M; Kuruvilla, Finny; Lagacé, Caroline; ... (2011). Deep resequencing of GWAS loci identifies independent rare variants associated with inflammatory bowel disease. Nature genetics, 43(11), pp. 1066-73. New York, N.Y.: Nature America 10.1038/ng.952

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More than 1,000 susceptibility loci have been identified through genome-wide association studies (GWAS) of common variants; however, the specific genes and full allelic spectrum of causal variants underlying these findings have not yet been defined. Here we used pooled next-generation sequencing to study 56 genes from regions associated with Crohn's disease in 350 cases and 350 controls. Through follow-up genotyping of 70 rare and low-frequency protein-altering variants in nine independent case-control series (16,054 Crohn's disease cases, 12,153 ulcerative colitis cases and 17,575 healthy controls), we identified four additional independent risk factors in NOD2, two additional protective variants in IL23R, a highly significant association with a protective splice variant in CARD9 (P < 1 × 10(-16), odds ratio ≈ 0.29) and additional associations with coding variants in IL18RAP, CUL2, C1orf106, PTPN22 and MUC19. We extend the results of successful GWAS by identifying new, rare and probably functional variants that could aid functional experiments and predictive models.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Gastro-intestinal, Liver and Lung Disorders (DMLL) > Clinic of Visceral Surgery and Medicine > Gastroenterology

UniBE Contributor:

Seibold, Frank Werner

ISSN:

1061-4036

Publisher:

Nature America

Language:

English

Submitter:

Factscience Import

Date Deposited:

04 Oct 2013 14:23

Last Modified:

17 Mar 2015 21:03

Publisher DOI:

10.1038/ng.952

PubMed ID:

21983784

Web of Science ID:

000296584000009

URI:

https://boris.unibe.ch/id/eprint/8214 (FactScience: 213720)

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