Anoxia tolerance in tobacco roots: effect of overexpression of pyruvate decarboxylase

Tadege, Million; Brändle, Roland; Kuhlemeier, Cris (1998). Anoxia tolerance in tobacco roots: effect of overexpression of pyruvate decarboxylase. The Plant Journal, 14(3), pp. 327-335. Blackwell Science 10.1046/j.1365-313X.1998.00130.x

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Plant survival during flooding relies on ethanolic fermentation for energy production. The available literature indicates that the first enzyme of the ethanolic fermentation pathway, pyruvate decarboxylase (PDC), is expressed at very low levels and is likely to be rate-limiting during oxygen deprivation. The authors expressed high levels of bacterial PDC in tobacco to study the modulation of PDC activity in vivo, and assess its impact on the physiology of ethanolic fermentation and survival under oxygen stress. In contrast to leaves, wild-type normoxic roots contained considerable PDC activity, and overexpression of the bacterial PDC caused only a moderate increase in acetaldehyde and ethanol production under anoxia compared to wild-type roots. No significant lactate production could be measured at any time, making it unlikely that lactate-induced acidification (LDH/PDC pH-stat) triggers the onset of ethanol synthesis. Instead, the authors favour a model in which the flux through the pathway is regulated by substrate availability. The increased ethanolic flux in the transgenics compared to the wild-type did not enhance anoxia tolerance. On the contrary, rapid utilisation of carbohydrate reserves enhanced premature cell death in the transgenics while replenishment of carbohydrates improved survival under anoxia.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Anoxia / Postanoxia (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Brändle, Roland and Kuhlemeier, Cris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0960-7412

Publisher:

Blackwell Science

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

02 Jun 2016 10:37

Last Modified:

14 Nov 2017 15:05

Publisher DOI:

10.1046/j.1365-313X.1998.00130.x

BORIS DOI:

10.7892/boris.82225

URI:

https://boris.unibe.ch/id/eprint/82225

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