NO2-induced nitrate reductase activity in needles of Norway spruce (Picea abies) under laboratory and field conditions

von Ballmoos, Peter; Ammann, Markus; Egger, Alfred; Suter, Marianne; Brunold, Christian (1998). NO2-induced nitrate reductase activity in needles of Norway spruce (Picea abies) under laboratory and field conditions. Physiologia Plantarum, 102(4), pp. 596-604. Blackwell 10.1034/j.1399-3054.1998.1020415.x

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The induction of activity of the enzyme nitrate reductase (NR, EC 1.6.6.1, 1.6.6.2) in needles of Norway spruce (Picea abies[L.] Karst.) by nitrogen dioxide (NO2) was studied under laboratory and field conditions. In fumigation chambers an increase in nitrate reductase activity (NRA) was detected 4 h after the start of the NO2 treatment. During the first 2 days with 100 µg NO2 m−3, NRA reached a constant level and did not change during the following 4 days. At the same level of NO2, NRA was lower in needles from trees grown on NPK-fertilized soil than on non-fertilized soil. After the transfer of spruce trees from fertilized soil to NPK-rich nutrient solution, NRA was transiently increased. This effect was assigned to root injuries causing nitrate transport to the shoot and subsequent induction of NRA. Neither trees on fertilized soil nor trees transferred to NPK-poor nutrient solution had increased NRA unless NO2 was provided. The NO2 gradient in the vicinity of a highway was used to test the long-term effect of elevated levels of NO2 on needle NRA of potted and field-grown spruce trees. Compared with less polluted sites, permanently increased NRAs were detected when NO2 concentrations were above 20 µg m−3. Controls of field measurements some 10 years after the introduction of catalytic converters in cars showed no significant change neither in NO2 levels nor in the decreasing NRA of spruce needles with the distance from the highway.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Stress Physiology (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

von Ballmoos, Peter; Ammann, Markus; Suter, Marianne and Brunold, Christian

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0031-9317

Publisher:

Blackwell

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

02 Jun 2016 10:03

Last Modified:

14 Nov 2017 16:29

Publisher DOI:

10.1034/j.1399-3054.1998.1020415.x

Uncontrolled Keywords:

Nitrate reductase activity, nitrogen dioxide, Norway spruce, Picea abies

BORIS DOI:

10.7892/boris.82229

URI:

https://boris.unibe.ch/id/eprint/82229

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