Hypersensitivity of an Arabidopsis Sugar Signaling Mutant toward Exogenous Proline Application

Hellmann, Hanjo; Funck, Dietmar; Rentsch, Doris; Frommer, Wolf B. (2000). Hypersensitivity of an Arabidopsis Sugar Signaling Mutant toward Exogenous Proline Application. Plant Physiology, 123(2), pp. 779-789. American Society of Plant Physiologists 10.1104/pp.123.2.779

[img] Text
PlantPhysiol_123_779.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

In transgenic Arabidopsis a patatin class I promoter from potato is regulated by sugars and proline (Pro), thus integrating signals derived from carbon and nitrogen metabolism. In both cases a signaling cascade involving protein phosphatases is involved in induction. Other endogenous genes are also regulated by both Pro and carbohydrates. Chalcone synthase (CHS) gene expression is induced by both, whereas the Pro biosynthetic Δ1-pyrroline-5-carboxylate synthetase (P5CS) is induced by high Suc concentrations but repressed by Pro, and Pro dehydrogenase (ProDH) is inversely regulated. The mutantrsr1-1, impaired in sugar dependent induction of the patatin promoter, is hypersensitive to low levels of external Pro and develops autofluorescence and necroses. Toxicity of Pro can be ameliorated by salt stress and exogenously supplied metabolizable carbohydrates. The rsr1-1 mutant shows a reduced response regarding sugar induction of CHS andP5CS expression. ProDH expression is de-repressed in the mutant but still down-regulated by sugar. Pro toxicity seems to be mediated by the degradation intermediate Δ1-pyrroline-5-carboxylate. Induction of the patatin promoter by carbohydrates and Pro, together with the Pro hypersensitivity of the mutant rsr1-1, demonstrate a new link between carbon/nitrogen and stress responses.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Molecular Plant Physiology
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Rentsch, Doris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0032-0889

Publisher:

American Society of Plant Physiologists

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

02 Jun 2016 11:52

Last Modified:

02 Jun 2016 11:52

Publisher DOI:

10.1104/pp.123.2.779

BORIS DOI:

10.7892/boris.82862

URI:

https://boris.unibe.ch/id/eprint/82862

Actions (login required)

Edit item Edit item
Provide Feedback