Direct electrospinning of 3D auricle-shaped scaffolds for tissue engineering applications.

Walser, Jochen; Stok, Kathryn S; Caversaccio, Marco; Ferguson, Stephen John (2016). Direct electrospinning of 3D auricle-shaped scaffolds for tissue engineering applications. Biofabrication, 8(2), 025007. IOP Publishing 10.1088/1758-5090/8/2/025007

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Thirty-two poly(ε)caprolactone (PCL) scaffolds have been produced by electrospinning directly into an auricle-shaped mould and seeded with articular chondrocytes harvested from bovine ankle joints. After seeding, the auricle shaped constructs were cultured in vitro and analysed at days 1, 7, 14 and 21 for regional differences in total DNA, glycosaminoglycan (GAG) and collagen (COL) content as well as the expression of aggrecan (AGG), collagen type I and type II (COL1/2) and matrix metalloproteinase 3 and 13 (MMP3/13). Stress-relaxation indentation testing was performed to investigate regional mechanical properties of the electrospun constructs. Electrospinning into a conductive mould yielded stable 3D constructs both initially and for the whole in vitro culture period, with an equilibrium modulus in the MPa range. Rapid cell proliferation and COL accumulation was observed until week 3. Quantitative real time PCR analysis showed an initial increase in AGG, no change in COL2, a persistent increase in COL1, and only a slight decrease initially for MMP3. Electrospinning of fibrous scaffolds directly into an auricle-shape represents a promising option for auricular tissue engineering, as it can reduce the steps needed to achieve an implantable structure.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Ear, Nose and Throat Disorders (ENT)
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute for Surgical Technology & Biomechanics ISTB
10 Strategic Research Centers > ARTORG Center for Biomedical Engineering Research > ARTORG Center - Image Guided Therapy > ARTORG Center - Artificial Hearing Research

UniBE Contributor:

Walser, Jochen; Caversaccio, Marco and Ferguson, Stephen John

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

1758-5090

Publisher:

IOP Publishing

Language:

English

Submitter:

Lars Marius Schwalbe

Date Deposited:

08 Jul 2016 13:40

Last Modified:

10 Jan 2017 14:56

Publisher DOI:

10.1088/1758-5090/8/2/025007

PubMed ID:

27171651

BORIS DOI:

10.7892/boris.82886

URI:

https://boris.unibe.ch/id/eprint/82886

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