Influence of nitrogen deficiency on senescence and the amounts of RNA and proteins in wheat leaves

Crafts-Brandner, Steven J.; Hölzer, Regina; Feller, Urs (1998). Influence of nitrogen deficiency on senescence and the amounts of RNA and proteins in wheat leaves. Physiologia Plantarum, 102(2), pp. 192-200. Blackwell 10.1034/j.1399-3054.1998.1020206.x

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Senescence-associated coordination in amounts of enzymes localized in different cellular compartments were determined in attached leaves of young wheat (Triticum aestivum L. cv. Arina) plants. Senescence was initiated at the time of full leaf elongation based on declines in total RNA and soluble protein. Removal of N from the growth medium just at the time of full leaf elongation enhanced the rate of senescence. Sustained declines in the amount of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, EC 4.1.1.39), and a marked decrease in the rbcS transcripts, just after full leaf elongation indicated that Rubisco synthesis/degradation was very sensitive to the onset of senescence. Rubisco activase amount also declined during senescence but the proportion of rca transcript relative to the total poly A RNA pool increased 3-fold during senescence. Thus, continued synthesis of activase may be required to maintain functional Rubisco throughout senescence. N stress led to declines in the amount of proteins located in the chloroplast, the peroxisome and the cytosol. Transcripts of the Clp protease subunits also declined in response to N stress, indicating that Clp is not a senescence-specific protease. In contrast to the other proteins, mitochondrial NADH-glutamate dehydrogenase (EC 1.4.1.2) was relatively stable during senescence and was not affected by N stress. During natural senescence with adequate plant nitrate supply the amount of nitrite reductase (EC 1.7.7.1) increased, and those of glutamine synthetase (EC 1.4.7.1) and glutamate synthase (EC 6.3.1.2) were stable. These results indicated that N assimilatory capacity can continue or even increase during senescence if the substrate supply is maintained. Differential stabilities of proteins, even within the same cellular compartment, indicate that proteolytic activity during senescence must be highly regulated.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant nutrition (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Hölzer, Regina and Feller, Urs

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0031-9317

Publisher:

Blackwell

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

13 Jul 2016 09:50

Last Modified:

13 Jul 2016 09:50

Publisher DOI:

10.1034/j.1399-3054.1998.1020206.x

Uncontrolled Keywords:

Carbon metabolism, Clp protease, nitrogen metabolism, senescence, Triticum aestivum, wheat

BORIS DOI:

10.7892/boris.83564

URI:

https://boris.unibe.ch/id/eprint/83564

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