Chlorophyll catabolism and gene expression in the peel of ripening banana fruits

Drury, Rosybel; Hörtensteiner, Stefan; Donnison, Iain; Bird, Colin R.; Seymour, Graham B. (1999). Chlorophyll catabolism and gene expression in the peel of ripening banana fruits. Physiologia Plantarum, 107(1), pp. 32-38. Blackwell 10.1034/j.1399-3054.1999.100105.x

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The biochemical and molecular basis of chlorophyll (Chl) catabolism in bananas was investigated during ripening at 20°C and at an elevated temperature (35°C) where degreening is inhibited. Biochemical analysis showed that Chl breakdown products could be isolated from fruit ripened at both temperatures. The coloured breakdown products, chlorophyllide and pheophorbide, were not detected at any stage of ripening in the two treatments; however, a non-fluorescent Chl catabolite accumulated to a higher concentration at 20 than at 35°C. To investigate the ripening-related gene expression associated with these changes, a cDNA library was generated from the peel of fruit ripened at 20°C. Differential screening of this library produced 20 non-redundant families of clones including those encoding enzymes involved in ethylene biosynthesis, respiration, starch metabolism, cell wall degradation and other metabolic events. The expression of these genes was followed by northern analysis in fruit ripened at 20 and 35°C.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant nutrition (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Hörtensteiner, Stefan

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0031-9317

Publisher:

Blackwell

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

13 Jul 2016 13:00

Last Modified:

13 Jul 2016 13:00

Publisher DOI:

10.1034/j.1399-3054.1999.100105.x

URI:

https://boris.unibe.ch/id/eprint/83567

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