Identification and characterization of GABA, proline and quaternary ammonium compound transporters from Arabidopsis thaliana

Breitkreuz, Kevin E.; Shelp, Barry J.; Fischer, Wolf N.; Schwacke, Rainer; Rentsch, Doris (1999). Identification and characterization of GABA, proline and quaternary ammonium compound transporters from Arabidopsis thaliana. FEBS letters, 450(3), 280 - 284. Elsevier 10.1016/S0014-5793(99)00516-5

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Arabidopsis thaliana grows efficiently on GABA as the sole nitrogen source, thereby providing evidence for the existence of GABA transporters in plants. Heterologous complementation of a GABA uptake-deficient yeast mutant identified two previously known plant amino acid transporters, AAP3 and ProT2, as GABA transporters with Michaelis constants of 12.9±1.7 and 1.7±0.3 mM at pH 4, respectively. The simultaneous transport of [1-14C]GABA and [2,3-3H]proline by ProT2 as a function of pH, provided evidence that the zwitterionic state of GABA is an important parameter in substrate recognition. ProT2-mediated [1-14C]GABA transport was inhibited by proline and quaternary ammonium compounds.

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