Identification and characterization of GABA, proline and quaternary ammonium compound transporters from Arabidopsis thaliana

Breitkreuz, Kevin E.; Shelp, Barry J.; Fischer, Wolf N.; Schwacke, Rainer; Rentsch, Doris (1999). Identification and characterization of GABA, proline and quaternary ammonium compound transporters from Arabidopsis thaliana. FEBS letters, 450(3), 280 - 284. Elsevier 10.1016/S0014-5793(99)00516-5

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Arabidopsis thaliana grows efficiently on GABA as the sole nitrogen source, thereby providing evidence for the existence of GABA transporters in plants. Heterologous complementation of a GABA uptake-deficient yeast mutant identified two previously known plant amino acid transporters, AAP3 and ProT2, as GABA transporters with Michaelis constants of 12.9±1.7 and 1.7±0.3 mM at pH 4, respectively. The simultaneous transport of [1-14C]GABA and [2,3-3H]proline by ProT2 as a function of pH, provided evidence that the zwitterionic state of GABA is an important parameter in substrate recognition. ProT2-mediated [1-14C]GABA transport was inhibited by proline and quaternary ammonium compounds.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Molecular Plant Physiology
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Rentsch, Doris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0014-5793

Publisher:

Elsevier

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

13 Jul 2016 08:50

Last Modified:

13 Jul 2016 08:50

Publisher DOI:

10.1016/S0014-5793(99)00516-5

Uncontrolled Keywords:

Arabidopsis thaliana, Amino acid transport, GABA; Proline, Zwitterionic, Quaternary ammonium compound

BORIS DOI:

10.7892/boris.83698

URI:

https://boris.unibe.ch/id/eprint/83698

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