LeProT1, a Transporter for Proline, Glycine Betaine, and γ-Amino Butyric Acid in Tomato Pollen

Schwacke, Rainer; Grallath, Silke; Breitkreuz, Kevin E.; Stransky, Elke; Stransky, Harald; Frommer, Wolf B.; Rentsch, Doris (1999). LeProT1, a Transporter for Proline, Glycine Betaine, and γ-Amino Butyric Acid in Tomato Pollen. The Plant Cell, 11(3), pp. 377-391. American Society of Plant Biologists 10.1105/tpc.11.3.377

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During maturation, pollen undergoes a period of dehydration accompanied by the accumulation of compatible solutes.Solute import across the pollen plasma membrane, which occurs via proteinaceous transporters, is required to support pollen development and also for subsequent germination and pollen tube growth. Analysis of the free amino acid composition of various tissues in tomato revealed that the proline content in flowers was 60 times higher than in any other organ analyzed. Within the floral organs, proline was confined predominantly to pollen, where it represented >70 of total free amino acids. Uptake experiments demonstrated that mature as well as germinated pollen rapidly take up proline. To identify proline transporters in tomato pollen, we isolated genes homologous to Arabidopsis proline transporters. LeProT1 was specifically expressed both in mature and germinating pollen, as demonstrated by RNA in situ hybridization. Expression in a yeast mutant demonstrated that LeProT1 transports proline and γ-amino butyric acid with low affinity and glycine betaine with high affinity. Direct uptake and competition studies demonstrate that LeProT1 constitutes a general transporter for compatible solutes.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Molecular Plant Physiology
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Rentsch, Doris

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

1040-4651

Publisher:

American Society of Plant Biologists

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

13 Jul 2016 08:57

Last Modified:

31 Mar 2017 11:44

Publisher DOI:

10.1105/tpc.11.3.377

BORIS DOI:

10.7892/boris.83699

URI:

https://boris.unibe.ch/id/eprint/83699

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