Production and characterisation of monoclonal antibodies specific for bovine interleukin-4

Weynants, V; Gilson, D; Furger, A; Collins, R A; Mertens, P; De Bolle, X; Heussler, Volker; Roditi, Isabel; Howard, C J; Dobbelaere, A E; Letesson, J J (1998). Production and characterisation of monoclonal antibodies specific for bovine interleukin-4. Veterinary immunology and immunopathology, 66(2), pp. 99-112. Elsevier 10.1016/S0165-2427(98)00184-6

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Genetic immunisation is a simple method for producing polyclonal antibodies in mice. By this method, we produced antibodies against bovine interleukin-4 (BoIL-4). After a final injection with a recombinant BoIL-4 protein, nine stable hybridoma cell lines were established which secreted monoclonal antibodies (MAbs) against this cytokine. Specific binding of each of the MAbs to recombinant BoIL-4 produced by Escherichia coli, baculovirus, and Trypanosoma brucei was demonstrated in an indirect ELISA and/or in Western blotting. These MAbs recognise the same antigenic region localised in the first 47 amino acids of the mature protein. None of them was able to neutralise the biological activity of the BoIL-4 under the conditions tested but one allowed the detection of BoIL-4 by flow cytometry.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology
08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria

UniBE Contributor:

Heussler, Volker and Roditi, Isabel

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

0165-2427

Publisher:

Elsevier

Language:

English

Submitter:

Volker Heussler

Date Deposited:

20 Jun 2016 11:11

Last Modified:

20 Jun 2016 11:11

Publisher DOI:

10.1016/S0165-2427(98)00184-6

PubMed ID:

9860184

BORIS DOI:

10.7892/boris.83753

URI:

https://boris.unibe.ch/id/eprint/83753

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