Genetic variability and limited clonality of Mycoplasma hyorhinis in pig herds

Trüeb, Bettina Salome; Catelli, Elena; Luehrs, Adrian; Nathues, Heiko; Kuhnert, Peter (2016). Genetic variability and limited clonality of Mycoplasma hyorhinis in pig herds. Veterinary microbiology, 191, pp. 9-14. Elsevier 10.1016/j.vetmic.2016.05.015

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Mycoplasma hyorhinis is a common inhabitant of the upper respiratory tract and tonsils of pigs. Its role as a possible pathogen remains controversial. In order to gain more insight into the epidemiology and population structure of M. hyorhinis we genetically characterized 60 isolates by multi locus sequence typing (MLST). The M. hyorhinis strains originated from Swiss and German pig herds with knowledge on the clinical background. The MLST scheme of Tocqueville et al. (J. Clin. Microbiol. 2014) was optimized, primers for the six MLST gene fragments were newly designed to allow amplification and sequencing with a single protocol. A total of 27 ST were observed with the 60 strains, 26 of those were previously unknown types. Generally identical genotypes were observed within a farm but they differed between farms. The identical genotype was also observed in three different Swiss farms. On the other Hand different genotypes within a farm were found with three German farms. The Swiss isolates formed a distinct cluster but otherwise there was no geographical nor a clinical association with specific Clusters observed. Data shows a high variability of M. hyorhinis comparable to what is observed for Mycoplasma hyopneumoniae. Similar to this pathogen the population structure of M. hyorhinis also shows some limited clonality with predominant genotypes within an animal and a single farm but different ones between farms. The comparable population structure of M. hyopneumoniae and M. hyorhinis could indicate a similar evolution of the two species in the common pig host.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management
05 Veterinary Medicine > Research Foci > Host-Pathogen Interaction
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV) > Swine Clinic
05 Veterinary Medicine > Department of Clinical Veterinary Medicine (DKV)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Veterinary Bacteriology

UniBE Contributor:

Trüeb, Bettina Salome; Catelli, Elena; Nathues, Heiko and Kuhnert, Peter

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0378-1135

Publisher:

Elsevier

Language:

English

Submitter:

Daniela Fasel

Date Deposited:

19 Jul 2016 10:45

Last Modified:

29 Oct 2019 18:58

Publisher DOI:

10.1016/j.vetmic.2016.05.015

PubMed ID:

27374901

Uncontrolled Keywords:

Coughing; Enzootic pneumonia; Genotyping; MLST; Pig disease

BORIS DOI:

10.7892/boris.83771

URI:

https://boris.unibe.ch/id/eprint/83771

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