Imaging liver-stage malaria parasites

Rankin, Kathleen E; Graewe, Stefanie; Heussler, Volker; Stanway, Rebecca R (2010). Imaging liver-stage malaria parasites. Cellular microbiology, 12(5), pp. 569-579. Blackwell 10.1111/j.1462-5822.2010.01454.x

[img] Text
j.1462-5822.2010.01454.x.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (509kB) | Request a copy

Plasmodium parasites, the causative agents of malaria, first invade and develop within hepatocytes before infecting red blood cells and causing symptomatic disease. Because of the low infection rates in vitro and in vivo, the liver stage of Plasmodium infection is not very amenable to biochemical assays, but the large size of the parasite at this stage in comparison with Plasmodium blood stages makes it accessible to microscopic analysis. A variety of imaging techniques has been used to this aim, ranging from electron microscopy to widefield epifluorescence and laser scanning confocal microscopy. High-speed live video microscopy of fluorescent parasites in particular has radically changed our view on key events in Plasmodium liver-stage development. This includes the fate of motile sporozoites inoculated by Anopheles mosquitoes as well as the transport of merozoites within merosomes from the liver tissue into the blood vessel. It is safe to predict that in the near future the application of the latest microscopy techniques in Plasmodium research will bring important insights and allow us spectacular views of parasites during their development in the liver.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Cell Biology > Malaria
08 Faculty of Science > Department of Biology > Institute of Cell Biology

UniBE Contributor:

Heussler, Volker

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

1462-5814

Publisher:

Blackwell

Language:

English

Submitter:

Volker Heussler

Date Deposited:

12 Jul 2016 08:35

Last Modified:

12 Jul 2016 08:35

Publisher DOI:

10.1111/j.1462-5822.2010.01454.x

PubMed ID:

20180802

BORIS DOI:

10.7892/boris.84089

URI:

https://boris.unibe.ch/id/eprint/84089

Actions (login required)

Edit item Edit item
Provide Feedback