Assessing stool quantities generated by three specific Kato-Katz thick smear templates employed in different settings.

Leuenberger, Andrea; Nassoro, Tatu; Said, Khadija; Fenner, Lukas; Sikalengo, George; Letang, Emilio; Montresor, Antonio; Zhou, Xiao-Nong; Steinmann, Peter; Marti, Hanspeter; Utzinger, Jürg; Knopp, Stefanie (2016). Assessing stool quantities generated by three specific Kato-Katz thick smear templates employed in different settings. Infectious diseases of poverty, 5(1), p. 58. BioMed Central 10.1186/s40249-016-0150-9

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The Kato-Katz technique is recommended for the diagnosis of helminth infections in epidemiological surveys, drug efficacy studies and monitoring of control interventions. We assessed the comparability of the average amount of faeces generated by three Kato-Katz templates included in test kits from two different providers.


Nine hundred Kato-Katz thick smear preparations were done; 300 per kit. Empty slides, slides plus Kato-Katz template filled with stool and slides plus stool after careful removal of the template were weighed to the nearest 0.1 mg. The average amount of stool that was generated on the slide was calculated for each template, stratified by standard categories of stool consistency (i.e. mushy, soft, sausage-shaped, hard and clumpy).


The average amount of stool generated on slides was 40.7 mg (95 % confidence interval (CI): 40.0-41.4 mg), 40.3 mg (95 % CI: 39.7-40.9 mg) and 42.8 mg (95 % CI: 42.2-43.3 mg) for the standard Vestergaard Frandsen template, and two different templates from the Chinese Center for Disease Control and Prevention (China CDC), respectively. Mushy stool resulted in considerably lower average weights when the Vestergaard Frandsen (37.0 mg; 95 % CI: 34.9-39.0 mg) or new China CDC templates (37.4 mg; 95 % CI: 35.9-38.9 mg) were used, compared to the old China CDC template (42.2 mg; 95 % CI: 40.7-43.7 mg) and compared to other stool consistency categories.


The average amount of stool generated by three specific Kato-Katz templates was similar (40.3-42.8 mg). Since the multiplication factor is somewhat arbitrary and small changes only have little effect on infection intensity categories, it is suggested that the standard multiplication factor of 24 should be kept for the calculation of eggs per gram of faeces for all investigated templates.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Social and Preventive Medicine (ISPM)

UniBE Contributor:

Fenner, Lukas


600 Technology > 610 Medicine & health
300 Social sciences, sociology & anthropology > 360 Social problems & social services




BioMed Central




Doris Kopp Heim

Date Deposited:

05 Jul 2016 11:49

Last Modified:

05 Dec 2022 14:57

Publisher DOI:


PubMed ID:


Uncontrolled Keywords:

Accuracy Diagnosis Infection intensity Kato-Katz technique Schistosomes Soil-transmitted helminths




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