Dynamic Precision Phenotyping Reveals Mechanism of Crop Tolerance to Root Herbivory

Qu, Wenchao; Robert, Christelle A. M.; Erb, Matthias; Hibbard, Bruce E.; Paven, Maxim; Gleede, Tassilo; Riehl, Barbara; Kersting, Lena; Cankaya, Aylin S.; Kunert, Anna T.; Xu, Youwen; Schueller, Michael J.; Shea, Colleen; Alexoff, David; Lee, SoJeong; Fowler, Joanna S.; Ferrieri, Richard A. (2016). Dynamic Precision Phenotyping Reveals Mechanism of Crop Tolerance to Root Herbivory. Plant Physiology, 172(2), pp. 776-788. American Society of Plant Physiologists 10.1104/pp.16.00735

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The western corn rootworm (WCR) is a major pest of maize that is well adapted to most crop management strategies. Breeding for tolerance is a promising alternative to combat WCR, but is currently constrained by a lack of physiological understanding and phenotyping tools. We developed dynamic precision phenotyping approaches using carbon-11 with positron emission tomography, root autoradiography and radiometabolite flux analysis to understand maize tolerance to WCR. Our results reveal that WCR attack induces specific patterns of lateral root growth which are associated with a shift in auxin biosynthesis from indole-3-pyruvic acid to indole-3-acetonitrile. WCR attack also increases transport of newly synthesized amino acids to the roots, including the accumulation of glutamine. Finally, the regrowth zones of WCR attacked roots show an increase in glutamine turnover which strongly correlates with the induction of indole-3-acetonitrile-dependent auxin biosynthesis. In summary, our findings identify local changes in the auxin flux network as a promising marker for induced WCR tolerance.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Biotic Interactions

UniBE Contributor:

Robert, Christelle Aurélie Maud and Erb, Matthias

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0032-0889

Publisher:

American Society of Plant Physiologists

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

22 Jul 2016 15:53

Last Modified:

28 Feb 2018 10:42

Publisher DOI:

10.1104/pp.16.00735

PubMed ID:

27406166

Uncontrolled Keywords:

Diabrotica virgifera virgifera; western corn rootworm; crop tolerance; dynamic precision phenotyping; carbon-11; auxin biosynthesis flux network; glutamine mobilization

BORIS DOI:

10.7892/boris.84555

URI:

https://boris.unibe.ch/id/eprint/84555

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