A Splice Defect in the EDA Gene in Dogs with an X-Linked Hypohidrotic Ectodermal Dysplasia (XLHED) Phenotype

Waluk, Dominik Pawel; Zur, Gila; Kaufmann, Ronnie; Welle, Monika Maria; Jagannathan, Vidhya; Drögemüller, Cord; Müller, Eliane Jasmine; Leeb, Tosso; Galichet, Arnaud (2016). A Splice Defect in the EDA Gene in Dogs with an X-Linked Hypohidrotic Ectodermal Dysplasia (XLHED) Phenotype. G3 Genes Genomes Genetics, 6(9), pp. 2949-2954. Genetics Society of America 10.1534/g3.116.033225

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X-linked hypohidrotic ectodermal dysplasia (XLHED) caused by variants in the EDA gene represents the most common ectodermal dysplasia in humans. We investigated three male mixed-breed dogs with an ectodermal dysplasia phenotype characterized by marked hypotrichosis and multifocal complete alopecia, almost complete absence of sweat and sebaceous glands and altered dentition with missing and abnormally shaped teeth. Analysis of SNP chip genotypes and whole genome sequence data from the three affected dogs revealed that the affected dogs shared the same haplotype on a large segment of the X-chromosome including the EDA gene. Unexpectedly, the whole genome sequence data did not reveal any non-synonymous EDA variant in the affected dogs. We therefore performed an RNA-seq experiment on skin biopsies to search for changes in the transcriptome. This analysis revealed that the EDA transcript in the affected dogs lacked 103 nucleotides encoded by exon 2. We speculate that this exon skipping is caused by a genetic variant located in one of the large introns flanking this exon, which was missed by whole genome sequencing with the illumina short read technology. The altered EDA transcript splicing most likely causes the observed ectodermal dysplasia in the affected dogs. These dogs thus offer an excellent opportunity to gain insights into the complex splicing processes required for expression of the EDA gene and other genes with large introns.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Dermatology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pathologie > Forschungsgruppe Dermatologie
05 Veterinary Medicine > Research Foci > DermFocus
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Institute of Genetics
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)

UniBE Contributor:

Waluk, Dominik Pawel; Welle, Monika Maria; Jagannathan, Vidya; Drögemüller, Cord; Müller, Eliane Jasmine; Leeb, Tosso and Galichet, Arnaud

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)

ISSN:

2160-1836

Publisher:

Genetics Society of America

Language:

English

Submitter:

Tosso Leeb

Date Deposited:

29 Aug 2016 08:13

Last Modified:

07 Feb 2017 11:05

Publisher DOI:

10.1534/g3.116.033225

PubMed ID:

27449516

Uncontrolled Keywords:

RNA-seq; canis familiaris; dog; splicing; whole genome sequencing

BORIS DOI:

10.7892/boris.85896

URI:

https://boris.unibe.ch/id/eprint/85896

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