Stem Cell-Associated Marker Expression in Canine Hair Follicles

Gerhards, Nora Merete; Sayar, Beyza; Origgi, Francesco; Galichet, Arnaud; Müller, Eliane Jasmine; Welle, Monika Maria; Wiener, Dominique Judith (2016). Stem Cell-Associated Marker Expression in Canine Hair Follicles. Journal of histochemistry and cytochemistry, 64(3), pp. 190-204. Sage 10.1369/0022155415627679

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Functional hair follicle (HF) stem cells (SCs) are crucial to maintain the constant recurring growth of hair. In mice and humans, SC subpopulations with different biomarker expression profiles have been identified in discrete anatomic compartments of the HF. The rare studies investigating canine HF SCs have shown similarities in biomarker expression profiles to that of mouse and human SCs. The aim of our study was to broaden the current repertoire of SC-associated markers and their expression patterns in the dog. We combined analyses on the expression levels of CD34, K15, Sox9, CD200, Nestin, LGR5 and LGR6 in canine skin using RT-qPCR, the corresponding proteins in dog skin lysates, and their expression patterns in canine HFs using immunohistochemistry. Using validated antibodies, we were able to define the location of CD34, Sox9, Keratin15, LGR5 and Nestin in canine HFs and confirm that all tested biomarkers are expressed in canine skin. Our results show similarities between the expression profile of canine, human and mouse HF SC markers. This repertoire of biomarkers will allow us to conduct functional studies and investigate alterations in the canine SC compartment of different diseases, like alopecia or skin cancer with the possibility to extend relevant findings to human patients.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Department of Dermatology, Urology, Rheumatology, Nephrology, Osteoporosis (DURN) > Clinic of Dermatology
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > Forschungsbereich Pathologie > Forschungsgruppe Dermatologie
05 Veterinary Medicine > Research Foci > DermFocus
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Institute of Animal Pathology
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP)
05 Veterinary Medicine > Department of Infectious Diseases and Pathobiology (DIP) > Center for Fish and Wildlife Health (FIWI)

UniBE Contributor:

Gerhards, Nora Merete; Sayar, Beyza; Origgi, Francesco; Galichet, Arnaud; Müller, Eliane Jasmine; Welle, Monika Maria and Wiener, Dominique Judith

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

0022-1554

Publisher:

Sage

Language:

English

Submitter:

Tosso Leeb

Date Deposited:

29 Aug 2016 09:11

Last Modified:

07 Feb 2017 11:07

Publisher DOI:

10.1369/0022155415627679

PubMed ID:

26739040

Uncontrolled Keywords:

Stem cells; canine; hair follicle; immunohistochemistry

BORIS DOI:

10.7892/boris.85979

URI:

https://boris.unibe.ch/id/eprint/85979

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