Insights into the in vitro Anticancer Effects of Diruthenium-1

Koceva-Chyła, Aneta; Matczak, Karolina; Hikisz, Msc. Paweł; Durka, Msc. Kamil; Kochel, Msc. Krzysztof; Süss-Fink, Georg; Furrer, Julien; Kowalski, Konrad (2016). Insights into the in vitro Anticancer Effects of Diruthenium-1. ChemMedChem, 11(19), pp. 2171-2187. Wiley-VCH 10.1002/cmdc.201600315

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The in vitro anticancer activity of the dinuclear trithiolato-bridged arene ruthenium complex diruthenium-1 (DiRu-1) was evaluated against a panel of human cancer cell lines used as in vitro models for hepatocellular carcinoma (HepG2 cells), estrogen-responsive breast adenocarcinoma (MCF-7 cells), and triple-negative breast adenocarcinoma (MDA-MB-231 cells). DiRu-1 is highly cytotoxic to these cell lines, demonstrating half-maximal inhibitory concentrations (IC50) in the low-nanomolar range (77±1.4 to 268.2±4.4 nm). The main molecular mechanisms responsible for the high cytotoxicity of DiRu-1 against the most responsive MCF-7 cell line (IC50=77±1.4 nm) were investigated on the basis of the capacity of DiRu-1 to induce oxidative stress, apoptosis, and DNA damage, and to inhibit the cell cycle and proliferation. The results show that DiRu-1 triggers caspase-dependent apoptosis in MCF-7 cells on both the intrinsic and extrinsic pathways. Moreover, the Ru complex also causes necrosis, mitotic catastrophe, and autophagy. DiRu-1 increases the intracellular levels of reactive oxygen species (ROS), which play a significant role in its cytotoxicity and pro-apoptotic activity. An important mechanism of the anticancer activity of DiRu-1 appears to be the induction of DNA lesions, mainly due to apoptotic DNA fragmentation and cell-cycle arrest at the G2/M checkpoint. These changes are correlated with the concentration of DiRu-1, the duration of the cell treatment, and the post-treatment time.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Departement of Chemistry and Biochemistry

UniBE Contributor:

Furrer, Julien

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 540 Chemistry

ISSN:

1860-7179

Publisher:

Wiley-VCH

Language:

English

Submitter:

Julien Henri Lucien Furrer

Date Deposited:

07 Sep 2016 11:02

Last Modified:

07 Oct 2016 01:31

Publisher DOI:

10.1002/cmdc.201600315

PubMed ID:

27561129

BORIS DOI:

10.7892/boris.87433

URI:

https://boris.unibe.ch/id/eprint/87433

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