Highly localised and persistent induction of Bx1 -dependent herbivore resistance factors in maize

Maag, Daniel; Köhler, Angela; Robert, Christelle A. M.; Frey, Monika; Wolfender, Jean-Luc; Turlings, Ted C.J.; Glauser, Gaétan; Erb, Matthias (2016). Highly localised and persistent induction of Bx1 -dependent herbivore resistance factors in maize. The Plant Journal, 88(6), pp. 976-991. Blackwell Science 10.1111/tpj.13308

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The induced production of secondary metabolites in herbivore-attacked plants varies in space and time. However, the consequences of these spatiotemporal patterns for herbivore performance are not well understood. This is particularly true for 1,4-benzoxazin-3-ones (BXs), the major induced defensive metabolites of maize. Here we report on the spatiotemporal dynamics of BX induction and its consequences for the leaf feeder Spodoptera littoralis. Defence-related phytohormones and transcript levels of BX biosynthetic genes were upregulated locally at the wound site within 12 hours of herbivory. Within another 12 hours, the insecticidal BX HDMBOA-Glc started to accumulate in a highly localised manner at the feeding site. Changes in BX metabolism away from the feeding site within the same leaf were much weaker and were undetected in systemic leaves. Following the removal of the caterpillars, local HDMBOA-Glc levels remained elevated for seven days. Caterpillars that were forced to feed directly on locally induced leaf parts, but not on adjacent leaf parts, suffered from reduced growth. This effect was abolished in the BX deficient bx1 mutant. We did not find any evidence that BXs regulate defensive phytohormones or their own accumulation. In summary, this study shows that induced herbivore resistance in maize is highly localised and dependent on BXs.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Biotic Interactions

UniBE Contributor:

Robert, Christelle Aurélie Maud and Erb, Matthias

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0960-7412

Publisher:

Blackwell Science

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

15 Sep 2016 09:03

Last Modified:

28 Feb 2018 10:41

Publisher DOI:

10.1111/tpj.13308

PubMed ID:

27538820

BORIS DOI:

10.7892/boris.87463

URI:

https://boris.unibe.ch/id/eprint/87463

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