Lateral root emergence in Arabidopsis is dependent on transcription factor LBD29 regulating auxin influx carrier LAX3

Porco, Silvana; Larrieu, Antoine; Du, Yujuan; Gaudinier, Allison; Goh, Tatsuaki; Swarup, Kamal; Swarup, Ranjan; Kuempers, Britta; Bishopp, Anthony; Lavenus, Julien; Casimiro, Ilda; Hill, Kristine; Benkova, Eva; Fukaki, Hidehiro; Brady, Siobhan M.; Scheres, Ben; Péret, Benjamin; Bennett, Malcolm J. (2016). Lateral root emergence in Arabidopsis is dependent on transcription factor LBD29 regulating auxin influx carrier LAX3. Development, 143(18), pp. 3340-3349. Company of Biologists Limited 10.1242/dev.136283

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Lateral root primordia (LRP) originate from pericycle stem cells located deep within parental root tissues. LRP emerge through overlying root tissues by inducing auxin-dependent cell separation and hydraulic changes in adjacent cells. The auxin inducible auxin influx carrier LAX3 plays a key role concentrating this signal in cells overlying LRP. Delimiting LAX3 expression to two adjacent cell files overlying new LRP is critical to ensure auxin-regulated cell separation occurs solely along their shared walls. Multiscale modeling has predicted this highly focused pattern of expression requires auxin to sequentially induce auxin efflux and influx carriers PIN3 and LAX3, respectively. Consistent with model predictions, we report that LAX3 auxin inducible expression is regulated indirectly by the AUXIN RESPONSE FACTOR ARF7. Yeast-1-hybrid screens revealed the LAX3 promoter is bound by the transcription factor LBD29, which is a direct target for regulation by ARF7. Disrupting auxin inducible LBD29 expression or expressing an LBD29-SRDX transcriptional repressor phenocopied the lax3 mutant, resulting in delayed lateral root emergence. We conclude that sequential LBD29 and LAX3 induction by auxin is required to coordinate cell separation and organ emergence.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant Development
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Lavenus, Julien

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0950-1991

Publisher:

Company of Biologists Limited

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

15 Sep 2016 07:58

Last Modified:

01 Sep 2017 02:30

Publisher DOI:

10.1242/dev.136283

PubMed ID:

27578783

BORIS DOI:

10.7892/boris.87774

URI:

https://boris.unibe.ch/id/eprint/87774

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