High-content analysis of factors affecting gold nanoparticle uptake by neuronal and microglial cells in culture

Stojiljkovic, Ana; Kuehni-Boghenbor, K.; Gaschen, Véronique; Schüpbach, Gertraud; Mevissen, Meike; Kinnear, C.; Möller, A.-M.; Stoffel, Michael Hubert (2016). High-content analysis of factors affecting gold nanoparticle uptake by neuronal and microglial cells in culture. Nanoscale, 8(37), pp. 16650-16661. Royal Society of Chemistry 10.1039/C6NR05723C

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Owing to their ubiquitous distribution, expected beneficial effects and suspected adverse effects, nanoparticles are viewed as a double-edged sword, necessitating a better understanding of their interactions with tissues and organisms. Thus, the goals of the present study were to develop and present a method to generate quantitative data on nanoparticle entry into cells in culture and to exemplarily demonstrate the usefulness of this approach by analyzing the impact of size, charge and various proteinaceous coatings on particle internalization. N9 microglial cells and both undifferentiated and differentiated SH-SY5Y neuroblastoma cells were exposed to customized gold nanoparticles. After silver enhancement, the particles were visualized by epipolarization microscopy and analysed by high-content analysis. The value of this approach was substantiated by assessing the impact of various parameters on nanoparticle uptake. Uptake was higher in microglial cells than in neuronal cells. Only microglial cells showed a distinct size preference, preferring particles with a diameter of 80 nm. Positive surface charge had the greatest impact on particle uptake. Coating with bovine serum albumin, fetuin or protein G significantly increased particle internalization in microglial cells but not in neuronal cells. Coating with wheat germ agglutinin increased particle uptake in both N9 and differentiated SH-SY5Y cells but not in undifferentiated SH-SY5Y cells. Furthermore, internalization was shown to be an active process and indicators of caspase-dependent apoptosis revealed that gold nanoparticles did not have any cytotoxic effects. The present study thus demonstrates the suitability of gold nanoparticles and high-content analysis for assessing numerous variables in a stringently quantitative and statistically significant manner. Furthermore, the results presented herein showcase the feasibility of specifically targeting nanoparticles to distinct cell types.

Item Type:

Journal Article (Original Article)

Division/Institute:

05 Veterinary Medicine > Research Foci > NeuroCenter
05 Veterinary Medicine > Research Foci > Veterinary Public Health / Herd Health Management
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Public Health Institute
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Pharmacology and Toxicology
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH) > Veterinary Anatomy
05 Veterinary Medicine > Department of Clinical Research and Veterinary Public Health (DCR-VPH)
09 Interdisciplinary Units > Microscopy Imaging Center MIC

UniBE Contributor:

Stojiljkovic, Ana; Gaschen, Véronique; Schüpbach, Gertraud; Mevissen, Meike and Stoffel, Michael Hubert

Subjects:

500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health

ISSN:

2040-3364

Publisher:

Royal Society of Chemistry

Language:

English

Submitter:

Miriam Francine Heinzelmann

Date Deposited:

27 Sep 2016 17:22

Last Modified:

11 Jan 2019 13:46

Publisher DOI:

10.1039/C6NR05723C

BORIS DOI:

10.7892/boris.88395

URI:

https://boris.unibe.ch/id/eprint/88395

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