A Broad RNA Virus Survey Reveals Both miRNA Dependence and Functional Sequestration.

Scheel, Troels K H; Luna, Joseph M; Liniger, Matthias; Nishiuchi, Eiko; Rozen-Gagnon, Kathryn; Shlomai, Amir; Auray, Gaël; Gerber, Markus; Fak, John; Keller, Irene; Bruggmann, Rémy; Darnell, Robert B; Ruggli, Nicolas; Rice, Charles M (2016). A Broad RNA Virus Survey Reveals Both miRNA Dependence and Functional Sequestration. Cell host & microbe, 19(3), pp. 409-423. Cell Press 10.1016/j.chom.2016.02.007

[img] Text
PIIS1931312816300488.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (6MB) | Request a copy

Small non-coding RNAs have emerged as key modulators of viral infection. However, with the exception of hepatitis C virus, which requires the liver-specific microRNA (miRNA)-122, the interactions of RNA viruses with host miRNAs remain poorly characterized. Here, we used crosslinking immunoprecipitation (CLIP) of the Argonaute (AGO) proteins to characterize strengths and specificities of miRNA interactions in the context of 15 different RNA virus infections, including several clinically relevant pathogens. Notably, replication of pestiviruses, a major threat to milk and meat industries, critically depended on the interaction of cellular miR-17 and let-7 with the viral 3' UTR. Unlike canonical miRNA interactions, miR-17 and let-7 binding enhanced pestivirus translation and RNA stability. miR-17 sequestration by pestiviruses conferred reduced AGO binding and functional de-repression of cellular miR-17 targets, thereby altering the host transcriptome. These findings generalize the concept of RNA virus dependence on cellular miRNAs and connect virus-induced miRNA sequestration to host transcriptome regulation.

Item Type:

Journal Article (Original Article)

UniBE Contributor:

Bruggmann, Rémy

ISSN:

1931-3128

Publisher:

Cell Press

Language:

English

Submitter:

Rémy Bruggmann

Date Deposited:

31 Dec 2019 07:32

Last Modified:

31 Dec 2019 07:40

Publisher DOI:

10.1016/j.chom.2016.02.007

PubMed ID:

26962949

Uncontrolled Keywords:

Argonaute CLIP-Seq HiTS-CLIP bovine viral diarrhea virus hepatitis C virus miRNA pestivirus sponge

BORIS DOI:

10.7892/boris.89005

URI:

https://boris.unibe.ch/id/eprint/89005

Actions (login required)

Edit item Edit item
Provide Feedback