Insight into the mechanism of transient trapping in micellar electrokinetic chromatography

Breadmore, Michael C; Quirino, Joselito P; Thormann, Wolfgang (2011). Insight into the mechanism of transient trapping in micellar electrokinetic chromatography. Electrophoresis, 32(5), pp. 542-9. Weinheim: Wiley-VCH 10.1002/elps.201000547

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Transient trapping is a new mechanism of on-line sample concentration and separation that has recently been presented. It involves the injection of a short length of micellar solution in front of the sample, making it similar to sweeping in partial-filling MEKC. Here, we examine the mechanism of transient trapping by the use of computer simulations and compare it to sweeping in MEKC for the two analytes, sulforhodamine B and 101. The simulation results confirm the mechanism for concentration and separation originally proposed. The mechanism for concentration is similar to sweeping since the analytes are picked and accumulated by the micelles that penetrate the sample zone. The mechanism for separation is however quite unique since the concentrated analytes are trapped for a few seconds on the sample/micelle boundary before they are released as the concentration of micelle is reduced as it undergoes electromigration dispersion and the analytes separate down a micelle gradient. Simulation results suggested that a significant contribution of band broadening arises from the micelle gradient, with shallower gradients resulting in broader peaks. However, this is offset by an increase in selectivity, such that resolution was enhanced even though the peaks are broader. Transient trapping analysis with similar resolution to those obtained by sweeping MEKC could be achieved in 1/10 of the time and 1/4 of the capillary length, which results in a 2-3 times increase in sensitivity.

Item Type:	Journal Article (Original Article)
Division/Institute:	04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Laboratory for Clinical Pharmacology
UniBE Contributor:	Thormann, Wolfgang
ISSN:	0173-0835
Publisher:	Wiley-VCH
Language:	English
Submitter:	Factscience Import
Date Deposited:	04 Oct 2013 14:25
Last Modified:	05 Dec 2022 14:07
Publisher DOI:	10.1002/elps.201000547
PubMed ID:	21341287
Web of Science ID:	000288094500008
URI:	https://boris.unibe.ch/id/eprint/8913 (FactScience: 214565)