Han, H-M; Zuber, Benoît; Dubochet, J (2008). Compression and crevasses in vitreous sections under different cutting conditions. Journal of microscopy, 230(Pt 2), pp. 167-171. Wiley-Blackwell 10.1111/j.1365-2818.2008.01972.x
![[img]](https://boris.unibe.ch/style/images/fileicons/text.png) |
Text
j.1365-2818.2008.01972.x.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (320kB) |
Compression and crevasses are common cutting artefacts in cryo-ultramicrotomy of vitreous sections. They can be reduced or suppressed under optimal cutting conditions. In the present study, compression and thickness were measured for different cutting speeds and knife angles. It was found that compression decreased with feed and that crevasses appeared only above a certain thickness. The optimal feed for vitreous sections was between 50 and 80 nm. The thickness, calculated by two independent methods, was quantitatively related to feed and compression.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy |
UniBE Contributor: |
Zuber, Benoît |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0022-2720 |
Publisher: |
Wiley-Blackwell |
Language: |
English |
Submitter: |
Benoît Zuber |
Date Deposited: |
14 Oct 2016 16:46 |
Last Modified: |
05 Dec 2022 14:59 |
Publisher DOI: |
10.1111/j.1365-2818.2008.01972.x |
PubMed ID: |
18445144 |
BORIS DOI: |
10.7892/boris.89303 |
URI: |
https://boris.unibe.ch/id/eprint/89303 |
Actions (login required)
 |
Edit item |