Compression and crevasses in vitreous sections under different cutting conditions.

Han, H-M; Zuber, Benoît; Dubochet, J (2008). Compression and crevasses in vitreous sections under different cutting conditions. Journal of microscopy, 230(Pt 2), pp. 167-171. Wiley-Blackwell 10.1111/j.1365-2818.2008.01972.x

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Compression and crevasses are common cutting artefacts in cryo-ultramicrotomy of vitreous sections. They can be reduced or suppressed under optimal cutting conditions. In the present study, compression and thickness were measured for different cutting speeds and knife angles. It was found that compression decreased with feed and that crevasses appeared only above a certain thickness. The optimal feed for vitreous sections was between 50 and 80 nm. The thickness, calculated by two independent methods, was quantitatively related to feed and compression.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy

UniBE Contributor:

Zuber, Benoît

Subjects:

600 Technology > 610 Medicine & health

ISSN:

0022-2720

Publisher:

Wiley-Blackwell

Language:

English

Submitter:

Benoît Zuber

Date Deposited:

14 Oct 2016 16:46

Last Modified:

14 Oct 2016 16:46

Publisher DOI:

10.1111/j.1365-2818.2008.01972.x

PubMed ID:

18445144

BORIS DOI:

10.7892/boris.89303

URI:

https://boris.unibe.ch/id/eprint/89303

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