Incorporation of atmospheric 15NO2-nitrogen into free amino acids by Norway spruce Picea abies (L.) Karst.

Nussbaum, Stefan; von Ballmoos, Peter; Gfeller, H.; Schlunegger, Urs Peter; Fuhrer, Jürg; Rhodes, D.; Brunold, Christian (1993). Incorporation of atmospheric 15NO2-nitrogen into free amino acids by Norway spruce Picea abies (L.) Karst. Oecologia, 94(3), pp. 408-414. Springer 10.1007/BF00317117

[img] Text
Oecologia_94_408.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (983kB) | Request a copy

During spring and autumn 1991, potted 6-yearold spruce trees (Picea abies (L.) Karst.) were fumigated with 60 nl1–1 15NO2 for 4 days under controlled conditions in constant light. Current and previous flush needles, the bark and the fine roots were analysed for total 15N content and incorporation of 15N into the -amino nitrogen of free amino acids. In addition, in vitro nitrate reductase activity and stomatal conductance of the needles were measured. Nitrate reductase activity was significantly higher in the needles of fumigated trees compared to control trees exposed to filtered air. With an average of 9.1% 15N, free glutamate was the pool with the most label. Taking into account the time-course of the labelling of this pool, this figure can be taken as an estimate of the minimum contribution of NO2 to the N nutrition of the needles. 15N-labelled amino acids were also detected in the bark and the roots, indicating export from the needles.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Stress Physiology (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

von Ballmoos, Peter; Schlunegger, Urs Peter; Fuhrer, Jürg and Brunold, Christian

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0029-8549

Publisher:

Springer

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

16 Nov 2016 10:50

Last Modified:

15 Nov 2017 14:46

Publisher DOI:

10.1007/BF00317117

Uncontrolled Keywords:

Amino acids, 15N incorporation, Nitrate reductase activity, Nitrogen dioxide, Picea abies

BORIS DOI:

10.7892/boris.89785

URI:

https://boris.unibe.ch/id/eprint/89785

Actions (login required)

Edit item Edit item
Provide Feedback