Wolbachia endosymbionts distort DNA barcoding in the parasitoid wasp genus Diplazon (Hymenoptera:Ichneumonidae)

Klopfstein, Seraina; Kropf, Christian; Baur, H (2016). Wolbachia endosymbionts distort DNA barcoding in the parasitoid wasp genus Diplazon (Hymenoptera:Ichneumonidae). Zoological Journal of the Linnean Society, 177(3), pp. 541-557. Wiley 10.1111/zoj.12380

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Molecular species delimitation has the potential to speed up both discovery and description rates for new species. However, several studies have shown a limited performance of the standard DNA barcoding approach which relies on a single mitochondrial gene, cytochrome oxidase 1 (COI). Besides incomplete lineage sorting or a low substitution rate, distortion of mitochondrial inheritance patterns by bacterial endosymbionts could explain the failure of barcoding. Numerous reviews have discussed this phenomenon, but only few empirical examples exist. In the present study, we examine the effect of Wolbachia bacteria on barcoding in the parasitoid wasp genus Diplazon. Although integrative taxonomy recognizes 16 species, COI only recovers up to ten. Adding multivariate morphometrics, genotyping a fast-evolving nuclear gene (ITS2) and screening the Diplazon species for Wolbachia, we show that the failure of DNA barcoding coincides with the presence of the endosymbiont. Two species even share identical COI haplotypes and Wolbachia strains, even though ITS2 suggests that they are not closely related. This is one of very few examples of mitochondrial DNA introgression between well-established insect species, facilitated by Wolbachia. We review similar reports, provide a list of criteria to identify endosymbiont-mediated introgression, and discuss the prevalence and impact of this phenomenon on insect systematics and evolution.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Ecology and Evolution (IEE) > Community Ecology

UniBE Contributor:

Klopfstein, Seraina and Kropf, Christian

Subjects:

500 Science > 570 Life sciences; biology
500 Science > 590 Animals (Zoology)
500 Science > 580 Plants (Botany)

ISSN:

1096-3642

Publisher:

Wiley

Language:

English

Submitter:

Alexander Strauss

Date Deposited:

17 Nov 2016 12:37

Last Modified:

17 Nov 2016 12:37

Publisher DOI:

10.1111/zoj.12380

BORIS DOI:

10.7892/boris.89939

URI:

https://boris.unibe.ch/id/eprint/89939

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