Mitochondrial growth during the cell cycle of Trypanosoma brucei bloodstream forms

Jakob, Martin; Hoffmann, Anneliese; Amodeo, Simona; Peitsch, Camille; Zuber, Benoît; Ochsenreiter, Torsten (2016). Mitochondrial growth during the cell cycle of Trypanosoma brucei bloodstream forms. Scientific Reports, 6(36565), p. 36565. Nature Publishing Group 10.1038/srep36565

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Mitochondrial organelles need to be replicated during cell division. Many aspects of this process have been studied in great detail, however the actual size increase and the position of organelle growth are less well understood. We use the protozoan parasite Trypanosoma brucei that contains a single mitochondrion to study organelle biogenesis by fluorescence microscopy. From the analysis of more than 1000 T. brucei bloodstream form cells of a nonsynchronous population we conclude that the mitochondrial network mostly grows from two areas along the main organelle axis, posterior and anterior of the nucleus. Loops and branches from these two areas eventually fuse to build a complex network. Together with the appearance of the division fold in the posterior part of the cell, pruning of the mitochondrial network and finally separation into the two daughter cells occurs. Overall organelle biogenesis is not continuous during cell growth and occurs mostly in the last part of the cell cycle. Furthermore, using 3D STED super resolution microscopy we reconstruct the volume of the organelle and characterize the region where the mitochondrial genome is positioned by serial block face scanning electron microscopy.

Item Type:

Journal Article (Original Article)


04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
08 Faculty of Science > Department of Biology > Institute of Cell Biology
09 Interdisciplinary Units > Microscopy Imaging Center (MIC)

Graduate School:

Graduate School for Cellular and Biomedical Sciences (GCB)

UniBE Contributor:

Jakob, Martin, Hoffmann, Anneliese, Amodeo, Simona, Zuber, Benoît, Ochsenreiter, Torsten


500 Science > 570 Life sciences; biology
600 Technology > 610 Medicine & health




Nature Publishing Group




Torsten Ochsenreiter

Date Deposited:

12 Dec 2016 09:39

Last Modified:

05 Dec 2022 15:00

Publisher DOI:


PubMed ID:





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