Li, Meiling; Lightfoot, Helen Louise; Halloy, Francois; Malinowska, Anna; Berk, Christian; Behera, Alok; Schümperli, Daniel; Hall, Jonathan (2017). Synthesis and cellular activity of stereochemically-pure 2’-O-(2-methoxyethyl)-phosphorothioate oligonucleotides. Chemical communications, 53(3), pp. 541-544. Royal Society of Chemistry 10.1039/C6CC08473G
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Stereochemically-pure 2’-O-(2-methoxyethyl)-phosphorothioate (PS-MOE) oligonucleotides were synthesized from new chiral oxazaphospholidine-containing nucleosides. Thermal stability studies showed that the incorporation of Rp-PS linkages increased RNA-binding affinity. In cells, a full Rp-PS-MOE splice-switching oligonucleotide targeting part of the ferrochelatase gene was more potent than its Sp-PS counterpart, but of similar potency to the stereorandom PS-parent sequence.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
08 Faculty of Science > Department of Biology > Institute of Cell Biology 08 Faculty of Science > Department of Biology > Institute of Cell Biology > RNA |
UniBE Contributor: |
Schümperli, Daniel |
Subjects: |
500 Science > 570 Life sciences; biology 500 Science > 540 Chemistry |
ISSN: |
1359-7345 |
Publisher: |
Royal Society of Chemistry |
Language: |
English |
Submitter: |
Daniel Schümperli |
Date Deposited: |
23 Jan 2017 14:04 |
Last Modified: |
05 Dec 2022 15:00 |
Publisher DOI: |
10.1039/C6CC08473G |
PubMed ID: |
27966701 |
BORIS DOI: |
10.7892/boris.91034 |
URI: |
https://boris.unibe.ch/id/eprint/91034 |
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