In-vitro activity of sodium-hypochlorite gel on bacteria associated with periodontitis.

Jurczyk, Karolina; Nietzsche, Sandor; Ender, Claudia; Sculean, Anton; Eick, Sigrun (2016). In-vitro activity of sodium-hypochlorite gel on bacteria associated with periodontitis. Clinical oral investigations, 20(8), pp. 2165-2173. Springer 10.1007/s00784-016-1711-9

Preview	Text In-vitro activity of sodium-hypochlorite gel on bacteria associated with periodontitis.pdf - Published Version Available under License Publisher holds Copyright. Download (3MB) | Preview
Preview	Text Perisolv_19Oct2015_clean.pdf - Accepted Version Available under License Publisher holds Copyright. Download (1MB) | Preview

OBJECTIVES

The aim of the present study was to assess the antimicrobial activity of a sodium hypochlorite formulation including its components against bacteria associated with periodontal disease.

MATERIALS AND METHODS

Sodium hypochlorite formulation (NaOCl gel), its components sodium hypochlorite (NaOCl), and the activating vehicle were compared with 0.1 % chlorhexidine digluconate (CHX) solution. The antimicrobial activity was proven by determination of minimal inhibitory concentrations (MIC), minimal bactericidal concentrations, and killing assays. Furthermore, the influence on formation as well as on a 4-day-old 6-species biofilm was tested.

RESULTS

Except for one strain (Parvimonas micra ATCC 33270 in case of NaOCl gel), the MICs both of the CHX solution and NaOCl gel did not exceed 10 % of the formulations' concentration. In general, MICs of the NaOCl gel were equal as of the CHX solution against Gram-negatives but higher against Gram-positive bacteria. CHX but not NaOCl gel clearly inhibited biofilm formation; however, the activity of NaOCl gel was more remarkable on a 4-day-old biofilm. NaOCl killed bacteria in the biofilm and interfered with the matrix.

CONCLUSIONS

The NaOCl gel acts antimicrobial in particular against Gram-negative species associated with periodontitis. Moreover, its component NaOCl hypochlorite is able to alter biofilm matrices.

CLINICAL RELEVANCE

The NaOCl gel may represent a potential alternative for adjunctive topical antimicrobial treatment in periodontitis.

Interest & Impact

Downloads

999 since deposited on 23 Jan 2017
121 in the past 12 months

Citations

5 Citations in Web of Science ®
6 Citations in Scopus

Search

in Google Scholar™

Services

Actions (login required)

Edit item

Actions (login required)

Edit item