Peracetic Acid Treatment Generates Potent Inactivated Oral Vaccines from a Broad Range of Culturable Bacterial Species.

Moor, Kathrin; Wotzka, Sandra Y; Toska, Albulena; Diard, Médéric; Hapfelmeier, Siegfried Hektor; Slack, Emma (2016). Peracetic Acid Treatment Generates Potent Inactivated Oral Vaccines from a Broad Range of Culturable Bacterial Species. Frontiers in immunology, 7(34), p. 34. Frontiers Research Foundation 10.3389/fimmu.2016.00034

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Our mucosal surfaces are the main sites of non-vector-borne pathogen entry, as well as the main interface with our commensal microbiota. We are still only beginning to understand how mucosal adaptive immunity interacts with commensal and pathogenic microbes to influence factors such as infectivity, phenotypic diversity, and within-host evolution. This is in part due to difficulties in generating specific mucosal adaptive immune responses without disrupting the mucosal microbial ecosystem itself. Here, we present a very simple tool to generate inactivated mucosal vaccines from a broad range of culturable bacteria. Oral gavage of 10(10) peracetic acid-inactivated bacteria induces high-titer-specific intestinal IgA in the absence of any measurable inflammation or species invasion. As a proof of principle, we demonstrate that this technique is sufficient to provide fully protective immunity in the murine model of invasive non-typhoidal Salmonellosis, even in the face of severe innate immune deficiency.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases > Research
04 Faculty of Medicine > Service Sector > Institute for Infectious Diseases

UniBE Contributor:

Hapfelmeier, Siegfried Hektor

Subjects:

500 Science > 570 Life sciences; biology

ISSN:

1664-3224

Publisher:

Frontiers Research Foundation

Language:

English

Submitter:

Siegfried Hektor Hapfelmeier-Balmer

Date Deposited:

06 Jan 2017 16:15

Last Modified:

11 Mar 2021 08:24

Publisher DOI:

10.3389/fimmu.2016.00034

PubMed ID:

26904024

Uncontrolled Keywords:

IgA; Salmonella typhimurium; Yersina enterocolytica; inactivated vaccines; oral vaccines

BORIS DOI:

10.7892/boris.91320

URI:

https://boris.unibe.ch/id/eprint/91320

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