L929 fibroblast bioassay on the in vitro toxicity of SnCl2, H3PO4, Clearfil SE primer and combinations thereof.

Schlüter, Nadine; Lussi, Adrian; Ganss, Carolina; Gruber, Reinhard (2016). L929 fibroblast bioassay on the in vitro toxicity of SnCl2, H3PO4, Clearfil SE primer and combinations thereof. Swiss dental journal, 126(6), pp. 566-572. Schweizerische Zahnärzte-Gesellschaft SSO

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Stannous chloride (SnCl(2), 35%) can increase microtensile bond strength between a self-etching adhesive and dental hard tissue either alone or in combination with phosphoric acid (H(3)PO(4), 35%). Whereas cell toxicity of H(3)PO(4) has been sufficiently investigated, little is known about the toxicity of concentrated SnCl(2). The present study determined the in vitro toxicity of SnCl(2), H(3)PO(4), the primer of a self-etching adhesive (Clearfil SE) and combinations thereof at three concentrations (0.01%-0.3%) in a L929 fibroblast bioassay. Cell viability was determined by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, the integrity of cell membranes was visualised by trypan blue staining (cell necrosis assay). Cell viability was impaired at concentrations between 0.09% and 0.13% for SnCl(2), between 0.06% and 0.09% for H(3)PO(4), and between 0.19% and 0.29% for Clearfil SE primer. Combinations of agents showed additive toxic effects. SnCl(2) showed a slightly lower but comparable in vitro toxicity to H(3)PO(4), which gives a perspective for further in vitro, in situ and clinical studies on this issue, and for the intraoral use of SnCl(2) to increase bond strength between an adhesive system and both enamel and dentine.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Restorative Dentistry, Research
04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research
04 Faculty of Medicine > School of Dental Medicine
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research

UniBE Contributor:

Schlüter, Nadine; Lussi, Adrian and Gruber, Reinhard

Subjects:

600 Technology > 610 Medicine & health

ISSN:

2296-6498

Publisher:

Schweizerische Zahnärzte-Gesellschaft SSO

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

02 Mar 2017 14:53

Last Modified:

16 Sep 2017 16:37

PubMed ID:

27377293

Uncontrolled Keywords:

L929; MTT; trypan blue; adhesive dentistry

BORIS DOI:

10.7892/boris.91532

URI:

https://boris.unibe.ch/id/eprint/91532

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