Galler, K M; Widbiller, M; Buchalla, W; Eidt, A; Hiller, K-A; Hoffer, P C; Schmalz, Gottfried Hans (2016). EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cells. International endodontic journal, 49(6), pp. 581-590. Wiley-Blackwell 10.1111/iej.12492
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EDTA conditioning of dentine promotes adhesion, migration and differentiation of dental pulp stem cellsl.pdf - Published Version Restricted to registered users only Available under License Publisher holds Copyright. Download (619kB) |
AIM
To evaluate the effect of dentine conditioning on migration, adhesion and differentiation of dental pulp stem cells.
METHODOLOGY
Dentine discs prepared from extracted human molars were pre-treated with EDTA (10%), NaOCl (5.25%) or H2 O. Migration of dental pulp stem cells towards pre-treated dentine after 24 and 48 h was assessed in a modified Boyden chamber assay. Cell adhesion was evaluated indirectly by measuring cell viability. Expression of mineralization-associated genes (COL1A1, ALP, BSP, DSPP, RUNX2) in cells cultured on pre-treated dentine for 7 days was determined by RT-qPCR. Nonparametric statistical analysis was performed for cell migration and cell viability data to compare different groups and time-points (Mann-Whitney U-test, α = 0.05).
RESULTS
Treatment of dentine with H2 O or EDTA allowed for cell attachment, which was prohibited by NaOCl with statistical significance (P = 0.000). Furthermore, EDTA conditioning induced cell migration towards dentine. The expression of mineralization-associated genes was increased in dental pulp cells cultured on dentine after EDTA conditioning compared to H2 O-pre-treated dentine discs.
CONCLUSIONS
EDTA conditioning of dentine promoted the adhesion, migration and differentiation of dental pulp stem cells towards or onto dentine. A pre-treatment with EDTA as the final step of an irrigation protocol for regenerative endodontic procedures has the potential to act favourably on new tissue formation within the root canal.
Item Type: |
Journal Article (Original Article) |
|---|---|
Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Department of Preventive, Restorative and Pediatric Dentistry |
UniBE Contributor: |
Schmalz, Gottfried Hans |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
0143-2885 |
Publisher: |
Wiley-Blackwell |
Language: |
English |
Submitter: |
Eveline Carmen Schuler |
Date Deposited: |
08 Mar 2017 09:06 |
Last Modified: |
05 Dec 2022 15:00 |
Publisher DOI: |
10.1111/iej.12492 |
PubMed ID: |
26114662 |
Uncontrolled Keywords: |
EDTA; dental pulp stem cells; dentine, growth factors; regenerative endodontics |
BORIS DOI: |
10.7892/boris.91570 |
URI: |
https://boris.unibe.ch/id/eprint/91570 |
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