Gene array of PDL cells exposed to Osteogain in combination with a bone grafting material.

Miron, Richard John; Shuang, Yuang; Sculean, Anton; Buser, Daniel; Chandad, Fatiha; Zhang, Yufeng (2016). Gene array of PDL cells exposed to Osteogain in combination with a bone grafting material. Clinical oral investigations, 20(8), pp. 2037-2043. Springer 10.1007/s00784-015-1702-2

[img] Text
Gene array of PDL cells exposed to Osteogain in combination with a bone grafting material.pdf - Published Version
Restricted to registered users only until 1 December 2021.
Available under License Publisher holds Copyright.

Download (299kB) | Request a copy

OBJECTIVES The aim of the present study was to investigate the effects of Osteogain, a new formulation of enamel matrix derivative (EMD) in combination with a grafting material on a wide variety of genes for cytokines, transcription factors and extracellular matrix proteins involved in osteoblast differentiation. MATERIALS AND METHODS Primary human periodontal ligament (PDL) cells were seeded on natural bone mineral (NBM) particles coated with Osteogain for 24 h and analyzed for regulated gene expression using a human osteogenesis gene super-array kit. Osteoblast-related genes include those transcribed during bone mineralization, ossification, bone metabolism, cell growth and differentiation as well as gene products representing extracellular matrix molecules, transcription factors and cell adhesion molecules. RESULTS Osteogain significantly upregulated the expression of over 20 of the 100 genes examined including bone morphogenetic protein 2 (BMP2), TGFβ1, fibroblast growth factor (FGF), epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) as well as some of their associated receptors. Osteogain also promoted gene expression of a number of osteoblast differentiation markers including collagen1α2 and alkaline phosphatase as well as cell adhesion molecules including fibronectin and a variety of integrin binding proteins. Interestingly, Osteogain promoted calcitonin receptor 55-fold and also promoted annexin A5 gene expression over 12-fold. CONCLUSION The present study demonstrates that Osteogain is capable of either upregulating or downregulating the expression of a wide variety of genes including those for growth factors and cytokines when combined with a bone grafting material. CLINICAL RELEVANCE The results from the present study demonstrate the large and potent effect of addition of Osteogain in combination to a bone grafting material over a wide variety of genes supporting osteogenesis.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology
04 Faculty of Medicine > School of Dental Medicine > Department of Oral Surgery and Stomatology
04 Faculty of Medicine > School of Dental Medicine
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Periodontics Research
04 Faculty of Medicine > School of Dental Medicine > School of Dental Medicine, Oral Surgery Research

UniBE Contributor:

Miron, Richard John; Sculean, Anton; Buser, Daniel and Zhang, Yufeng

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1432-6981

Publisher:

Springer

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

08 Mar 2017 10:38

Last Modified:

08 Mar 2017 10:38

Publisher DOI:

10.1007/s00784-015-1702-2

PubMed ID:

26744181

Uncontrolled Keywords:

Bone graft; Emdogain; Enamel matrix derivative; Enamel matrix Proteins; Periodontal regeneration

BORIS DOI:

10.7892/boris.91607

URI:

https://boris.unibe.ch/id/eprint/91607

Actions (login required)

Edit item Edit item
Provide Feedback