In vitro effects of 0 to 120 Grays of irradiation on bone viability and release of growth factors.

Sawada, Kosaku; Kobayashi, Masako; Kobayashi, Eizaburo; Brömme, Jens; Schaller, Benoît; Miron, Richard John (2016). In vitro effects of 0 to 120 Grays of irradiation on bone viability and release of growth factors. BMC Oral Health, 17(1)(4), p. 4. BioMed Central 10.1186/s12903-016-0241-9

[img]
Preview
Text
In vitro effects of 0 to 120 Grays of irradiation on bone viability and release of growth factors.pdf - Published Version
Available under License Creative Commons: Attribution (CC-BY).

Download (1MB) | Preview

BACKGROUND High dose radiation therapy is commonly used in maxillofacial surgeries to treat a number of head and neck tumors. Despite its widespread use, little information is available regarding the effects of irradiation on bone cell viability and release of growth factors following dose-dependent irradiation. METHODS Bone samples were collected from porcine mandibular cortical bone and irradiated at doses of 0, 7.5, 15, 30, 60 and 120 Grays. Thereafter, cell viability was quantified, and the release of growth factors including TGFβ1, BMP2, VEGF, IL1β and RANKL were investigated over time. RESULTS It was observed that at only 7.5Gy of irradiation, over 85 % of cells were non-vital and by 60 Gy, all cells underwent apoptosis. Furthermore, over a 7-fold decrease in VEGF and a 2-fold decrease in TGFβ1 were observed following irradiation at all tested doses. Little change was observed for BMP2 and IL1β whereas RANKL was significantly increased for all irradiated samples. CONCLUSIONS These results demonstrate the pronounced effects of irradiation on bone-cell vitality and subsequent release of growth factors. Interestingly, the largest observed change in gene expression was the 7-fold decrease in VEGF protein following irradiation. Future research aimed at improving our understanding of bone following irradiation is necessary to further improve future clinical treatments.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie
04 Faculty of Medicine > Pre-clinic Human Medicine > BioMedical Research (DBMR) > DBMR Forschung Mu35 > Forschungsgruppe Schädel-, Kiefer- und Gesichtschirurgie

04 Faculty of Medicine > Department of Head Organs and Neurology (DKNS) > Clinic of Craniomaxillofacial Surgery
04 Faculty of Medicine > Department of Haematology, Oncology, Infectious Diseases, Laboratory Medicine and Hospital Pharmacy (DOLS) > Clinic of Radiation Oncology

UniBE Contributor:

Sawada, Kosaku; Kobayashi, Masako; Kobayashi, Eizaburo; Brömme, Jens; Schaller, Benoît and Miron, Richard John

Subjects:

600 Technology > 610 Medicine & health

ISSN:

1472-6831

Publisher:

BioMed Central

Language:

English

Submitter:

Eveline Carmen Schuler

Date Deposited:

26 Jan 2017 16:37

Last Modified:

07 Feb 2017 15:21

Publisher DOI:

10.1186/s12903-016-0241-9

PubMed ID:

27431387

Uncontrolled Keywords:

Bone cell death, Bone Chips, Grays Growth factor release, Irradiation

BORIS DOI:

10.7892/boris.91609

URI:

https://boris.unibe.ch/id/eprint/91609

Actions (login required)

Edit item Edit item
Provide Feedback