Zhang, Yufeng; Wei, Lingfei; Miron, Richard John; Shi, Bin; Bian, Zhuan (2016). Bone scaffolds loaded with siRNA-Semaphorin4d for the treatment of osteoporosis related bone defects. Scientific Reports, 6(26925), p. 26925. Nature Publishing Group 10.1038/srep26925
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Bone scaffolds loaded with siRNA-Semaphorin4d for the treatment of osteoporosis related bone defects.pdf - Published Version Available under License Creative Commons: Attribution (CC-BY). Download (2MB) | Preview |
Osteoporosis is a prominent disorder affecting over 200 million people worldwide. Recently, semaphorins have been implicated in the cell-cell communication between osteoclasts and osteoblasts and have been associated with the progression of osteoporosis. Previously, we demonstrated that knockdown of semaphorin4d (Sema4d) using siRNA delivered with a bone-targeting system prevented bone loss in an osteoporotic animal model. Here, we used this bone-specific technology containing siRNA-Sema4d and fabricated a PLLA scaffold capable of enhancing bone repair following fracture. We investigated the ability of the implant to release siRNA-Sema4d into the surrounding tissues over time and to influence new bone formation in a 3 mm femur osteoporotic defect model in ovariectomized rats. Delivery of the bone-targeting system released from PLLA scaffolds began 2 hours post-implantation, peaked at 1 day, and was sustained over a 21 day period. μCT analysis demonstrated a significantly higher bone volume/total volume bone mineral density and number of osteoblasts in the rats that were transplanted with scaffolds loaded with siRNA-Sema4d. These results confirm the specific role of Sema4d in bone remodeling and demonstrate that significant increases in the speed and quality of new bone formation occur when siRNA-Sema4d is delivered via a PLLA scaffold.
Item Type: |
Journal Article (Original Article) |
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Division/Institute: |
04 Faculty of Medicine > School of Dental Medicine > Restorative Dentistry, Research 04 Faculty of Medicine > School of Dental Medicine > Periodontics Research 04 Faculty of Medicine > School of Dental Medicine > Oral Surgery Research 04 Faculty of Medicine > School of Dental Medicine > Department of Periodontology 04 Faculty of Medicine > School of Dental Medicine |
UniBE Contributor: |
Zhang, Yufeng, Miron, Richard John |
Subjects: |
600 Technology > 610 Medicine & health |
ISSN: |
2045-2322 |
Publisher: |
Nature Publishing Group |
Language: |
English |
Submitter: |
Eveline Carmen Schuler |
Date Deposited: |
02 Feb 2017 16:30 |
Last Modified: |
05 Dec 2022 15:00 |
Publisher DOI: |
10.1038/srep26925 |
PubMed ID: |
27254469 |
BORIS DOI: |
10.7892/boris.91611 |
URI: |
https://boris.unibe.ch/id/eprint/91611 |
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