Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication.

Baum, Oliver; Torchetti, Eleonora; Malik, Corinna; Hoier, Birgitte; Walker, Meegan; Walker, Philip J; Odriozola Quesada, Adolfo; Graber, Franziska; Tschanz, Stefan A.; Bangsbo, Jens; Hoppeler, Hans-Heinrich; Askew, Christopher D; Hellsten, Ylva (2016). Capillary ultrastructure and mitochondrial volume density in skeletal muscle in relation to reduced exercise capacity of patients with intermittent claudication. American journal of physiology - regulatory, integrative and comparative physiology, 310(10), R943-R951. American Physiological Society 10.1152/ajpregu.00480.2015

[img] Text
R943.full.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

Intermittent claudication (IC) is the most commonly reported symptom of peripheral arterial disease (PAD). Impaired limb blood flow is a major casual factor of lower exercise tolerance in PAD but cannot entirely explain it. We hypothesized that IC is associated with structural changes of the capillary-mitochondria interface that could contribute to the reduction of exercise tolerance in IC patients. Capillary and mitochondrial morphometry were performed after light and transmission electron microscopy using vastus lateralis muscle biopsies of 14 IC patients and 10 age-matched controls, and peak power output (PPO) was determined for all participants using an incremental single-leg knee-extension protocol. Capillary density was lower (411 ± 90 mm(-2) vs. 506 ± 95 mm(-2); P ≤ 0.05) in the biopsies of the IC patients than in those of the controls. The basement membrane (BM) around capillaries was thicker (543 ± 82 nm vs. 423 ± 97 nm; P ≤ 0.01) and the volume density of mitochondria was lower (3.51 ± 0.56% vs. 4.60 ± 0.74%; P ≤ 0.01) in the IC patients than the controls. In the IC patients, a higher proportion of capillaries appeared with collapsed slit-like lumen and/or swollen endothelium. PPO was lower (18.5 ± 9.9 W vs. 33.5 ± 9.4 W; P ≤ 0.01) in the IC patients than the controls. We suggest that several structural alterations in skeletal muscle, either collectively or separately, contribute to the reduction of exercise tolerance in IC patients.

Item Type:

Journal Article (Original Article)

Division/Institute:

04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy
04 Faculty of Medicine > Pre-clinic Human Medicine > Institute of Anatomy > Functional Anatomy
04 Faculty of Medicine > Service Sector > Institute of Legal Medicine > Forensic Medicine
09 Interdisciplinary Units > Microscopy Imaging Center MIC

UniBE Contributor:

Baum, Oliver; Torchetti, Eleonora; Odriozola Quesada, Adolfo; Graber, Franziska; Tschanz, Stefan A. and Hoppeler, Hans-Heinrich

Subjects:

600 Technology > 610 Medicine & health
500 Science > 570 Life sciences; biology

ISSN:

0363-6119

Publisher:

American Physiological Society

Language:

English

Submitter:

David Christian Haberthür

Date Deposited:

27 Dec 2016 16:40

Last Modified:

14 Feb 2019 14:00

Publisher DOI:

10.1152/ajpregu.00480.2015

PubMed ID:

27009051

Uncontrolled Keywords:

capillary; morphometry; peripheral arterial disease; skeletal muscle; transmission electron microscopy

BORIS DOI:

10.7892/boris.91738

URI:

https://boris.unibe.ch/id/eprint/91738

Actions (login required)

Edit item Edit item
Provide Feedback