Impact of post-anoxia stress on membrane lipids of anoxia-pretreated potato cells. A re-appraisal

Pavelic, Danijela; Arpagaus, Silvio; Rawyler, André; Brändle, Roland (2000). Impact of post-anoxia stress on membrane lipids of anoxia-pretreated potato cells. A re-appraisal. Plant Physiology, 124(3), pp. 1285-1292. American Society of Plant Physiologists 10.1104/pp.124.3.1285

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The importance of lipid peroxidation and its contributing pathways (via reactive oxygen species and lipoxygenase) during post-anoxia was evaluated with respect to the biphasic behavior of membrane lipids under anoxia (A. Rawyler, D. Pavelic, C. Gianinazzi, J. Oberson, R. Brandle 1999] Plant Physiol 120: 293-300), using potato (Solanum tuberosum cv Bintje) cell cultures. When anoxic cells in the pre-lytic phase were re-oxygenated for 2 h, superoxide anion was not detectable, the hydrogen peroxide (H2O2) level remained small and similar to that of controls, and cell viability was presented. Lipids were intact and no lipid hydroperoxides were detected. However, small amounts of lipid hydroperoxides accumulated upon feeding anoxic cells with H2O2 and incubation for an additional 2 h under anoxia. When cells that entered the lytic phase of anoxia were re-oxygenated for 2 h, the H2O2 and superoxide anion levels were essentially unchanged. However, cell respiration decreased, reflecting the extensive lipid hydrolysis that had already started under anoxia and continued during post-anoxia. Simultaneous with the massive release of free polyunsaturated fatty acids, small amounts of lipid hydroperoxides were formed, reaching 1% to 2%; of total fatty acids. Catalase and superoxide dismutase activities were not greatly affected, whereas the amount and activity of lipoxygenase tended to increase during anoxia. Lipid peroxidation in potato cells is therefore low during post-anoxia. It is mainly due to lipoxygenase, whereas the contribution of reactive oxygen species is negligible. But above all, it is a late event that occurs only when irreversible damage is already caused by the anoxia-triggered lipid hydrolysis.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Anoxia / Postanoxia [discontinued]
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Brändle, Roland

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0032-0889

Publisher:

American Society of Plant Physiologists

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

07 Mar 2017 08:17

Last Modified:

14 Nov 2017 14:53

Publisher DOI:

10.1104/pp.124.3.1285

BORIS DOI:

10.7892/boris.91760

URI:

https://boris.unibe.ch/id/eprint/91760

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