Occurrence and characteristics of the mitochondrial permeability transition in plants

Arpagaus, Silvio; Rawyler, André; Brändle, Roland (2002). Occurrence and characteristics of the mitochondrial permeability transition in plants. Journal of biological chemistry, 277(3), pp. 1780-1787. American Society for Biochemistry and Molecular Biology 10.1074/jbc.M109416200

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dThe behavior of purified potato mitochondria toward the main effectors of the animal mitochondrial permeability transition has been studied by light scattering, fluorescence, SDS-polyacrylamide gel electrophoresis, and immunoblotting techniques. The addition of Ca2+ induces a phosphate-dependent swelling that is fully inhibited by cyclosporin A if dithioerythritol is present. Mg2+ cannot be substituted for Ca2+ but competes with it. Disruption of the outer membrane and release of several proteins, including cytochrome c, occur upon completion of swelling. Ca2+-induced swelling is delayed and its rate is decreased when pH is shifted from 7.4 to 6.6. It is accelerated by diamide, phenylarsine oxide, and linolenic acid. In the absence of Ca2+, however, linolenic acid (less than or equal to20 mum) rapidly dissipates the succinate-driven membrane potential while having no effect on mitochondrial volume. Anoxic conditions favor in vitro swelling and the concomitant release of cytochrome c and of other proteins in a pH-dependent way. These data indicate that the classical mitochondrial permeability transition occurs also in plants. This may have important implications for our understanding of cell stress and death processes.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Anoxia / Postanoxia [discontinued]
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Brändle, Roland

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0021-9258

Publisher:

American Society for Biochemistry and Molecular Biology

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

06 Jul 2017 11:55

Last Modified:

14 Nov 2017 14:51

Publisher DOI:

10.1074/jbc.M109416200

PubMed ID:

11704674

BORIS DOI:

10.7892/boris.91791

URI:

https://boris.unibe.ch/id/eprint/91791

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