Light-induced proteolysis of stromal proteins in pea (Pisum sativum L.) chloroplasts: requirement for intact organelles

Roulin, Samuel; Feller, Urs (1997). Light-induced proteolysis of stromal proteins in pea (Pisum sativum L.) chloroplasts: requirement for intact organelles. Plant science, 128(1), pp. 31-41. Elsevier 10.1016/S0168-9452(97)00140-4

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In intact chloroplasts isolated from mature pea leaves (Pision sativum L.), the catabolism of ribulose-1,5-bisphosphate carboxylase/oxygenase as well as several other stromal proteins was strongly accelerated by illumination. However, when proteolysis was compared in intact and lysed chloroplasts this rapid, light-induced degradation of stromal proteins was markedly reduced following lysis of the organelles. Varying the pH of the incubation medium in a physiologically relevant range or adding adequate concentrations of ATP or Mg2+ could not restore rapid protein breakdown in lysed chloroplasts. Using an approach combining fluorography, Coomassie-staining and immunoblotting, the stability of several stromal proteins in intact and lysed organelles could be monitored simultaneously in the same chloroplast preparation. Evidence is presented that the light-induced proteolysis is a genuine intraplastidial process. (C) 1997 Elsevier Science Ireland Ltd.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant nutrition (discontinued)
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Feller, Urs

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0168-9452

Publisher:

Elsevier

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

06 Jul 2017 14:23

Last Modified:

13 Jun 2018 17:51

Publisher DOI:

10.1016/S0168-9452(97)00140-4

Uncontrolled Keywords:

light-induced proteolysis, stromal proteins, pea; Pisum sativum L, chloroplast intactness

BORIS DOI:

10.7892/boris.91875

URI:

https://boris.unibe.ch/id/eprint/91875

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