Protein catabolism in bean leaf discs: accumulation of a soluble fragment of ribulose-1,5-bisphosphate carboxylase/oxygenase under oxygen deficiency

Hildbrand, Markus; Fischer, Andreas; Feller, Urs (1994). Protein catabolism in bean leaf discs: accumulation of a soluble fragment of ribulose-1,5-bisphosphate carboxylase/oxygenase under oxygen deficiency. Journal of Experimental Botany, 45(9), pp. 1197-1204. Oxford University Press 10.1093/jxb/45.9.1197

[img] Text
1994_JExpBot_45_1197.pdf - Published Version
Restricted to registered users only
Available under License Publisher holds Copyright.

Download (1MB) | Request a copy

Discs isolated from the primary leaves of 2-week-old bean plants were used to study the influence of different light intensities (between 0 and 27 mu E m(-2) s(-1) on Rubisco and chlorophyll catabolism. In discs with a water-infiltrated intercellular space, the rate of chlorophyll degradation strongly depended on the light intensity, while illumination was far less effective in non-infiltrated leaf discs. It has been suggested in the past that stromal proteins can be cleaved in the intact organelle. In our experiments, a 45 kDa Rubisco breakdown product accumulated in infiltrated leaf discs in darkness (where chlorophyll was stable) or after an incubation under N-2. One or several steps involved in the further degradation of this catabolite might be oxygen- or energy-dependent. The solubility of Rubisco and of the fragment accumulating under hypoxia in the dark was investigated, since an association of this enzyme with membranes under certain conditions has been reported in the past. As judged from SDS-PAGE gels, neither the intact large subunit nor the fragment accumulated in the membrane fraction in our experimental system. The intermediates formed during Rubisco degradation might depend on environmental conditions. At present, the steps involved in the catabolism of this enzyme cannot be generalized.

Item Type:

Journal Article (Original Article)

Division/Institute:

08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS) > Plant nutrition [discontinued]
08 Faculty of Science > Department of Biology > Institute of Plant Sciences (IPS)

UniBE Contributor:

Feller, Urs

Subjects:

500 Science > 580 Plants (Botany)

ISSN:

0022-0957

Publisher:

Oxford University Press

Language:

English

Submitter:

Peter Alfred von Ballmoos-Haas

Date Deposited:

05 Jul 2017 09:02

Last Modified:

05 Jul 2017 09:02

Publisher DOI:

10.1093/jxb/45.9.1197

Uncontrolled Keywords:

rubisco, proteolysis, senescence, leaf disc, bean

BORIS DOI:

10.7892/boris.91887

URI:

https://boris.unibe.ch/id/eprint/91887

Actions (login required)

Edit item Edit item
Provide Feedback